Addition of DNase improves the in vitro activity of antifungal drugs against Candida albicans biofilms.
Addition of DNase improves the in vitro activity of antifungal drugs against Candida albicans biofilms.
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DOI：
10.1111/j.1439-0507.2011.02047.x
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发表时间：
2012-01
期刊：
影响因子：
4.9
通讯作者：
Oliveira R
中科院分区：
文献类型：
--
作者：
Martins M;Henriques M;Lopez-Ribot JL;Oliveira R
白色念珠菌生物膜内的细胞对大多数临床使用的抗真菌药物的敏感性降低。我们最近证明，细胞外DNA(EDNA)作为生物膜基质的一个组成部分，在生物膜完整性中发挥着重要作用。通过研究脱氧核糖核酸酶I(DNase)与抗真菌药物联用对白色念珠菌生物被膜的影响，了解EDNA对白色念珠菌生物被膜抗真菌敏感性的影响。白色念珠菌生物膜的形成使用简单和可重复性的96孔板为基础的方法。采用2，3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium氢氧化物还原试验、活菌计数法检测0.13 mg L−1脱氧核糖核酸酶与抗真菌药物联用的活性。在这里，我们报告了两性霉素B与DNA酶联合使用时对白色念珠菌生物被膜的改善效果，通过XTT读数和活菌计数进行评估。此外，尽管DNA酶提高了卡泊芬净降低线粒体活性的效果，但在可培养细胞方面没有观察到变化。DNA酶不影响生物膜细胞对氟康唑的敏感性。这项工作表明，以影响生物膜结构完整性的过程为靶点的药物可能有潜在的辅助导管锁定治疗的用途。
Cells within Candida albicans biofilms display decreased susceptibility to most clinically used antifungal agents. We recently demonstrated that extracellular DNA (eDNA) plays an important role in biofilm integrity, as a component of the biofilm matrix. To gain insight into the contributions of eDNA to C. albicans biofilms antifungal susceptibility by the investigation of the impact of the combined use of deoxyribonuclease I (DNase) and antifungals to treat biofilms. C. albicans biofilms were formed using a simple and reproducible 96-well plate-based method. The activity of the combined use of 0.13 mg l−1 DNase and antifungals was estimated by the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay, and total viable counts. Here we report the improved efficacy of amphotericin B when in combination with DNase against C. albicans biofilms, as assessed by XTT readings and viable counts. Furthermore, although DNase increased the efficacy of caspofungin in the reduction of mitochondrial activity, no changes were observed in terms of culturable cells. DNase did not affect biofilm cells susceptibility to fluconazole. This work suggests that agents that target processes affecting the biofilm structural integrity may have potential use as adjuvants of a catheter–lock therapy.
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影响因子：
3.6
作者：
Allesen-Holm, M;Barken, KB;Tolker-Nielsen, T
通讯作者：
Tolker-Nielsen, T
影响因子：
4.9
作者：
Bachmann, SP;VandeWalle, K;López-Ribot, JL
通讯作者：
López-Ribot, JL
影响因子：
4.9
作者：
Vediyappan, Govindsamy;Rossignol, Tristan;d'Enfert, Christophe
通讯作者：
d'Enfert, Christophe
DOI：
10.1086/651200
发表时间：
2010-07-01
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Nett JE;Sanchez H;Cain MT;Andes DR
通讯作者：
Andes DR
影响因子：
4.9
作者：
Ramage, G;vande Walle, K;López-Ribot, JL
通讯作者：
López-Ribot, JL