Reducing dietary loading decreases mouse temporomandibular joint degradation induced by anterior crossbite prosthesis.
Reducing dietary loading decreases mouse temporomandibular joint degradation induced by anterior crossbite prosthesis.
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DOI：
10.1016/j.joca.2013.11.014
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发表时间：
2014-02
影响因子：
7
通讯作者：
Wang, M. -Q.
中科院分区：
文献类型：
--
作者：
Liu, Y. -D.;Liao, L. -F.;Zhang, H. -Y.;Lu, L.;Jiao, K.;Zhang, M.;Zhang, J.;He, J. -J.;Wu, Y. -P.;Chen, D.;Wang, M. -Q.
饮食负荷可通过牙周肌反射影响颞下颌关节（TMJ）的改建。因此，我们研究是否减少饮食负荷减少颞下颌关节退化引起的单侧前牙反牙合修复，我们最近报道。将40只6周龄雌性C57 BL/6 J小鼠随机分为两个实验组和两个对照组。一个实验组和一个对照组接受小尺寸饮食，另外两个组接受大尺寸饮食。两组均制作单侧前牙反牙合修复体。术后3周取颞下颌关节标本。用苏木精-伊红、甲苯胺蓝、番红O和抗酒石酸酸性磷酸酶染色观察髁突软骨和软骨下骨的组织学变化。采用实时PCR和/或免疫组织化学方法检测TMJ髁突软骨和/或软骨下骨中II型胶原、聚集蛋白聚糖、具有血小板反应蛋白基序5的去整合素和金属蛋白酶（ADAMTS-5）以及RANKL/RANK/OPG的表达水平。两个实验组的TMJ中观察到软骨变薄和退化，软骨细胞密度降低，II型胶原和聚集蛋白聚糖表达水平降低，软骨下骨丢失和破骨细胞活性增强。但与大规格组相比，小规格组软骨降解表型较轻，软骨ADAMTS-5 mRNA表达较低，软骨和软骨下骨中OPG/RANKL比值较高。两对照组之间的组织形态学和受试分子无差异。目前的研究结果表明，通过提供小尺寸饮食来降低功能负荷水平可以减少由异常咬合引起的生物力学刺激引起的TMJ降解。
Dietary loading has been reported to have an effect on temporomandibular joint (TMJ) remodeling via periodontal-muscular reflex. We therefore examined whether reducing dietary loading decreased TMJ degradation induced by the unilateral anterior crossbite prosthesis as we recently reported. Forty 6-week-old female C57BL/6J mice were randomly divided into two experimental and two control groups. One experimental and one control group received small-size diet and the other two groups received large-size diet. Unilateral anterior crossbite prosthesis was created in the two experimental groups. The TMJ samples were collected 3 weeks after experimental operation. Histological changes in condylar cartilage and subchondral bone were assessed by Hematoxylin & Eosin, toluidine blue, Safranin O and tartrate-resistant acid phosphatase staining. Real-time polymerase chain reaction (PCR) and/or immunohistochemistry were performed to evaluate the expression levels of Collagen II, Aggrecan, a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS-5) and RANKL/RANK/OPG in TMJ condylar cartilage and/or subchondral bone. Thinner and degraded cartilage, reduced cartilage cellular density, decreased expression levels of Collagen II and Aggrecan, loss of subchondral bone and enhanced osteoclast activity were observed in TMJs of both experimental groups. However, the cartilage degradation phenotype was less severe and cartilage ADAMTS-5 mRNA was lower while OPG/RANKL ratio in cartilage and subchondral bone was higher in the small-size than large-size diet experimental group. No differences of histomorphology and the tested molecules were found between the two control groups. The current findings suggest that a lower level of functional loading by providing small-size diet could reduce TMJ degradation induced by the biomechanical stimulation from abnormal occlusion.
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通讯作者：
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