Sex differences in chondrocyte maturation in the mandibular condyle from a decreased occlusal loading model.
Sex differences in chondrocyte maturation in the mandibular condyle from a decreased occlusal loading model.
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DOI：
10.1007/s00223-011-9498-9
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发表时间：
2011-08
影响因子：
4.2
通讯作者：
Wadhwa, S.
中科院分区：
文献类型：
--
作者：
Chen, J.;Sobue, T.;Utreja, A.;Kalajzic, Z.;Xu, M.;Kilts, T.;Young, M.;Wadhwa, S.
颞下颌关节紊乱（TMDs）主要困扰育龄妇女。机械负荷引起的颞下颌关节（TMJ）重塑缺陷被认为是TMD发展的主要病因。本研究的目的是确定CD-1和C57BL/6小鼠暴露于减少咬合负荷的TMJ重塑模型中是否存在性别差异。21日龄的CD-1、C57BL/6雌雄小鼠各分为两组。他们被喂食正常的颗粒饲料（正常负荷）或软饲料，并将他们的门牙修剪出咬合（减少咬合负荷）4周。对髁突软骨进行组织学检查，对软骨下骨进行显微ct检查。用实时荧光定量PCR分析两组基因表达情况。在这两个品系和性别的小鼠中，与正常负荷对照组相比，减少咬合负荷对软骨下骨的影响相似，骨量和总体积减少。然而，在这两种菌株中，与正常负荷对照组相比，减少咬合负荷仅在雌性小鼠中导致II型胶原（Col2）和Sox9的表达显著降低，而在雄性小鼠中则没有。咬合负荷的减少导致两性骨体积的减少，并且仅在雌性小鼠中导致早期软骨细胞成熟的减少。
Temporomandibular joint disorders (TMDs) predominantly afflict women of childbearing age. Defects in mechanical loading-induced temporomandibular joint (TMJ) remodeling are believed to be a major etiological factor in the development of TMD. The goal of this study was to determine if there are sex differences in CD-1 and C57BL/6 mice exposed to a decreased occlusal loading TMJ remodeling model. Male and female CD-1 and C57BL/6 mice, 21 days old, were each divided into two groups. They were fed either a normal pellet diet (normal loading) or a soft diet and had their incisors trimmed out of occlusion (decreased occlusal loading) for 4 weeks. The mandibular condylar cartilage was evaluated by histology, and the subchondral bone was evaluated by micro-CT analysis. Gene expression from both was evaluated by real-time PCR analysis. In both strains and sexes of mice, decreased occlusal loading caused similar effects in the subchondral bone, decreases in bone volume and total volume compared with their normal loading controls. However, in both strains, decreased occlusal loading caused a significant decrease in the expression of collagen type II (Col2) and Sox9 only in female mice, but not in male mice, compared with their normal loading controls. Decreased occlusal loading causes decreased bone volume in both sexes and a decrease in early chondrocyte maturation exclusively in female mice.
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