Multiplexed Profiling of Extracellular Vesicles for Biomarker Development.
Multiplexed Profiling of Extracellular Vesicles for Biomarker Development.
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生物标志物发展中细胞外囊泡的多路分析。
DOI：
10.1007/s40820-021-00753-w
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发表时间：
2021-12-02
影响因子：
26.6
通讯作者：
Tofaris GK
中科院分区：
文献类型：
--
作者：
Jiang C;Fu Y;Liu G;Shu B;Davis J;Tofaris GK
细胞外囊泡（EV）多路复用涉及基于化学、物理空间、生物或纳米颗粒的策略。EV生物标志物开发中的多路复用包括高通量筛选以及到目前为止主要应用于EV表面蛋白或内部货物miRNA的即时检测平台。单EV分辨率的多重测量可能会彻底改变EV分析物作为复杂和异质性疾病生物标志物的适用性。细胞外囊泡（EV）是细胞来源的膜颗粒，在分子运输、细胞间运输和不需要的蛋白质的排出中起着至关重要的作用。它们与许多疾病有关，包括癌症和神经变性。EV在所有体液中都能检测到，它们的蛋白质和核酸含量提供了一种评估它们起源的细胞状态的方法。因此，它们为生物标志物发现提供了机会，用于疾病的诊断、预后或分层以及对治疗的客观监测。有效的实际应用需要同时测定多种EV衍生的标志物，并且多路复用平台已经发展出实现这一点的潜力。在此，我们提供了一个全面的概述，目前可用的多路复用平台的EV分析，主要集中在小型化和集成设备，提供潜在的阶跃变化的分析能力，吞吐量和一致性。
Extracellular vesicle (EV) multiplexing involves chemical, physical spatial, biological, or nanoparticle-based strategies. Multiplexing in EV biomarker development includes high-throughput screening as well as point-of-care testing platforms which to date have been applied mainly to EV surface proteins or internal cargo miRNAs. Multiplexed measurements at single-EV resolution are likely to revolutionize the applicability of EV analytes as biomarkers in complex and heterogeneous diseases. Extracellular vesicles (EVs) are cell-derived membranous particles that play a crucial role in molecular trafficking, intercellular transport and the egress of unwanted proteins. They have been implicated in many diseases including cancer and neurodegeneration. EVs are detected in all bodily fluids, and their protein and nucleic acid content offers a means of assessing the status of the cells from which they originated. As such, they provide opportunities in biomarker discovery for diagnosis, prognosis or the stratification of diseases as well as an objective monitoring of therapies. The simultaneous assaying of multiple EV-derived markers will be required for an impactful practical application, and multiplexing platforms have evolved with the potential to achieve this. Herein, we provide a comprehensive overview of the currently available multiplexing platforms for EV analysis, with a primary focus on miniaturized and integrated devices that offer potential step changes in analytical power, throughput and consistency.
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