Rapid Isolation and Multiplexed Detection of Exosome Tumor Markers Via Queued Beads Combined with Quantum Dots in a Microarray.
Rapid Isolation and Multiplexed Detection of Exosome Tumor Markers Via Queued Beads Combined with Quantum Dots in a Microarray.
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通过排队珠与微阵列中的量子点相结合快速分离和多重检测外泌体肿瘤标志物
DOI：
10.1007/s40820-019-0285-x
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发表时间：
2019-07-16
影响因子：
26.6
通讯作者：
Mao H
中科院分区：
文献类型：
--
作者：
Bai Y;Lu Y;Wang K;Cheng Z;Qu Y;Qiu S;Zhou L;Wu Z;Liu H;Zhao J;Mao H
开发了用于外泌体分离和多重肿瘤标志物检测的基于珠的微阵列。微珠被均匀地捕获并排列在芯片中的微柱之间，这可以避免光学干扰并实现更准确的测试结果。不同类型肺癌外泌体样品的结果显示出不同的标记物表达水平。本文的在线版本（10.1007/s40820-019-0285-x）包含补充材料，可供授权用户使用。肿瘤来源的外泌体积极参与癌症的进展和转移，并已成为液体活检中癌症诊断的有前途的标志物。由于它们的纳米尺寸，复杂的生物起源以及与外泌体分离和检测相关的方法学限制，它们的分析进展仍然缓慢。微流控技术提供了一个更好的分析方法相比，传统的方法。在这里，我们开发了一种基于珠的微阵列，用于外泌体分离和多重肿瘤标志物检测。使用这种方法，通过与珠子表面的抗体结合来分离外泌体，并通过量子点（QD）探针检测外泌体上的肿瘤标志物。然后，珠子被均匀地捕获并在芯片中的微柱中排队。这种设计通过将信号分散到每个微珠中而有利于荧光观察，从而避免光学干扰并实现更准确的测试结果。我们分析了肺癌和内皮细胞系的细胞培养上清液中的外泌体，并使用三种QD探针标记的不同肺癌标记物来进行外泌体表面蛋白标记物的多重检测。肺癌来源的样品显示出比内皮细胞样品高得多的荧光强度（约六倍至十倍），并且不同类型的肺癌样品显示出不同的标记物表达水平。此外，使用该芯片检测来自癌症患者的临床血浆样品显示出良好的诊断能力，并显示出与常规血清学标记物检测的良好一致性。这些结果为外泌体肿瘤标志物检测和早期癌症诊断提供了一种有前途的方法。 本文的在线版本（10.1007/s40820-019-0285-x）包含补充材料，可供授权用户使用。
A bead-based microarray for exosome isolation and multiplexed tumor marker detection was developed. The beads are uniformly trapped and queued among the micropillars in the chip, which can avoid optical interference and enable more accurate test results. The results with different types of lung cancer exosome samples showed distinctive marker expression levels. The online version of this article (10.1007/s40820-019-0285-x) contains supplementary material, which is available to authorized users. Tumor-derived exosomes are actively involved in cancer progression and metastasis and have emerged as a promising marker for cancer diagnosis in liquid biopsy. Because of their nanoscale size, complex biogenesis, and methodological limitations related to exosome isolation and detection, advancements in their analysis remain slow. Microfluidic technology offers a better analytic approach compared with conventional methods. Here, we developed a bead-based microarray for exosome isolation and multiplexed tumor marker detection. Using this method, exosomes are isolated by binding to antibodies on the bead surface, and tumor markers on the exosomes are detected through quantum dot (QD) probes. The beads are then uniformly trapped and queued among micropillars in the chip. This design benefits fluorescence observation by dispersing the signals into every single bead, thereby avoiding optical interference and enabling more accurate test results. We analyzed exosomes in the cell culture supernatant of lung cancer and endothelial cell lines, and different lung cancer markers labeled with three QD probes were used to conduct multiplexed detection of exosome surface protein markers. Lung cancer-derived samples showed much higher (~ sixfold–tenfold) fluorescence intensity than endothelial cell samples, and different types of lung cancer samples showed distinctive marker expression levels. Additionally, using the chip to detect clinical plasma samples from cancer patients showed good diagnostic power and revealed a well consistency with conventional tests for serological markers. These results provide insight into a promising method for exosome tumor marker detection and early-stage cancer diagnosis. The online version of this article (10.1007/s40820-019-0285-x) contains supplementary material, which is available to authorized users.
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影响因子：
26.6
作者：
Li R;Zhou M;Li J;Wang Z;Zhang W;Yue C;Ma Y;Peng H;Wei Z;Hu Z
通讯作者：
Hu Z
影响因子：
26.6
作者：
Shikha S;Zheng X;Zhang Y
通讯作者：
Zhang Y
影响因子：
6.1
作者：
Chen C;Skog J;Hsu CH;Lessard RT;Balaj L;Wurdinger T;Carter BS;Breakefield XO;Toner M;Irimia D
通讯作者：
Irimia D
影响因子：
14.9
作者：
Mathivanan S;Fahner CJ;Reid GE;Simpson RJ
通讯作者：
Simpson RJ
影响因子：
6.1
作者：
Contreras-Naranjo JC;Wu HJ;Ugaz VM
通讯作者：
Ugaz VM