Overexpression of ANXA1 in penile carcinomas positive for high-risk HPVs.
Overexpression of ANXA1 in penile carcinomas positive for high-risk HPVs.
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DOI：
10.1371/journal.pone.0053260
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Rahal P
中科院分区：
文献类型：
--
作者：
Calmon MF;Mota MT;Babeto É;Candido NM;Girol AP;Mendiburu CF;Bonilha JL;Silvestre RV;Rosa BM;Thomé JA;Medeiros GH;Soares FA;Guimarães GC;de Arruda JG;Oliani SM;Villa LL;Vassallo J;Rahal P
阴茎癌的发病率因人群而异，但在发达国家很少见。阴茎癌与许多已确定的风险因素和相关疾病有关，包括包茎伴慢性炎症、人乳头瘤病毒（HPV）感染、卫生不良和吸烟。这项研究的目的是确定与这种类型的癌症相关的基因。对47例阴茎鳞状细胞癌标本进行HPV检测分析。48.9%的阴茎鳞状细胞癌组织中HPV DNA阳性。42.5%的阴茎鳞状细胞癌中存在高危型HPV，10.6%的阴茎鳞状细胞癌中检测到低危型HPV。RaSH方法鉴定了高危HPV阳性阴茎癌中膜联蛋白A1（ANXA 1）、p16、RPL 6、PBEF 1和KIAA 1033的差异表达;通过qPCR，与正常阴茎样本相比，高危HPV阳性阴茎鳞状细胞中ANXA 1和p16过表达。与HPV阴性肿瘤相比，ANXA 1和p16蛋白在高危HPV阳性阴茎癌细胞中的表达显著增加（p<0.0001），与癌的亚型无关。ANXA 1在高危型HPV感染者阴茎鳞癌中的过表达可能是由HPV E6介导的，提示该基因在阴茎癌的发生中起重要作用。
The incidence of penile cancer varies between populations but is rare in developed nations. Penile cancer is associated with a number of established risk factors and associated diseases including phimosis with chronic inflammation, human papillomavirus (HPV) infection, poor hygiene and smoking. The objective of this study was to identify genes related to this type of cancer. The detection of HPV was analyzed in 47 penile squamous cell carcinoma samples. HPV DNA was detected in 48.9% of penile squamous cell carcinoma cases. High-risk HPV were present in 42.5% of cases and low-risk HPV were detected in 10.6% of penile squamous cell carcinomas. The RaSH approach identified differential expression of Annexin A1 (ANXA1), p16, RPL6, PBEF1 and KIAA1033 in high-risk HPV positive penile carcinoma; ANXA1 and p16 were overexpressed in penile squamous cells positive for high-risk HPVs compared to normal penile samples by qPCR. ANXA1 and p16 proteins were significantly more expressed in the cells from high-risk HPV-positive penile carcinoma as compared to HPV-negative tumors (p<0.0001) independently of the subtype of the carcinoma. Overexpression of ANXA1 might be mediated by HPV E6 in penile squamous cell carcinoma of patients with high-risk HPVs, suggesting that this gene plays an important role in penile cancer.
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影响因子：
4.8
作者：
Alldridge, LC;Harris, HJ;Bryant, CE
通讯作者：
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影响因子：
3.7
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发表时间：
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作者：
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通讯作者：
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影响因子：
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作者：
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