Visceral pressure stimulator for exploring hollow organ pain: a pilot study.
Visceral pressure stimulator for exploring hollow organ pain: a pilot study.
复制标题
DOI：
10.1186/s12938-021-00870-y
复制
发表时间：
2021-03-25
影响因子：
3.9
通讯作者：
Kolber BJ
中科院分区：
文献类型：
--
作者：
DeLong M;Gil-Silva M;Hong VM;Babyok O;Kolber BJ
压力刺激的调节和控制对许多疼痛和伤害性感觉的研究是有用的，特别是在内脏疼痛领域。在许多活体实验中，不同压力的不同气体和液体刺激被输送到中空器官，如膀胱、阴道和结肠。这些刺激与对刺激的反应的行为、分子或生理读出相结合。在实验过程中，必须注意提供精确的定时刺激。例如，刺激信号可以在线使用，以精确地将刺激与生理输出进行时间锁定。这种精确度要求开发专门的硬件来控制刺激(例如空气)，同时提供压力和刺激信号标记的精确读出。在这项研究中，我们设计了一个定时压力调节器[称为内脏压力刺激器(VPS)]来控制空气流量，测量压力(以毫米汞为单位)，并将刺激标记发送到在线软件。该装置是用一个简单的电路和主要现成的部件制造的。使用单独的定制直联式模数压力转换器来验证VPS的实际压力输出。使用商业生理学软件(Spike2，CED)，我们能够在给予小鼠独特的空气刺激试验期间连续测量小鼠的膀胱压力，同时记录上腹部肌肉的肌电(EMG)。对于那些需要(1)提供不同的压力刺激，同时(2)实时测量压力和(3)使用生理软件监控刺激开关的人来说，该设备将是有用的。网上版载有补充材料，可在10.1186/s12938-021-00870-y查阅。
The regulation and control of pressure stimuli is useful for many studies of pain and nociception especially those in the visceral pain field. In many in vivo experiments, distinct air and liquid stimuli at varying pressures are delivered to hollow organs such as the bladder, vagina, and colon. These stimuli are coupled with behavioral, molecular, or physiological read-outs of the response to the stimulus. Care must be taken to deliver precise timed stimuli during experimentation. For example, stimuli signals can be used online to precisely time-lock the stimulus with a physiological output. Such precision requires the development of specialized hardware to control the stimulus (e.g., air) while providing a precise read-out of pressure and stimulus signal markers. In this study, we designed a timed pressure regulator [termed visceral pressure stimulator (VPS)] to control air flow, measure pressure (in mmHg), and send stimuli markers to online software. The device was built using a simple circuit and primarily off-the-shelf parts. A separate custom inline analog-to-digital pressure converter was used to validate the real pressure output of the VPS. Using commercial physiological software (Spike2, CED), we were able to measure mouse bladder pressure continuously during delivery of unique air stimulus trials in a mouse while simultaneously recording an electromyogram (EMG) of the overlying abdominal muscles. This device will be useful for those who need to (1) deliver distinct pressure stimuli while (2) measuring the pressure in real-time and (3) monitoring stimulus on–off using physiological software. The online version contains supplementary material available at 10.1186/s12938-021-00870-y.
登录
查看更多内容
影响因子：
6.6
作者：
Ness, TJ;Elhefni, H
通讯作者：
Elhefni, H
影响因子：
1.2
作者：
Sadler, Katelyn E.;Stratton, Jarred M.;Kolber, Benedict J.
通讯作者：
Kolber, Benedict J.
影响因子：
2.3
作者：
Cervero, Fernando
通讯作者：
Cervero, Fernando
影响因子：
3.7
作者：
Sadler, Katelyn E.;Stratton, Jarred M.;Kolber, Benedict J.
通讯作者：
Kolber, Benedict J.
DOI：
10.1111/luts.12365
发表时间：
2021-04
期刊：
Lower urinary tract symptoms
影响因子：
--
作者：
Srivastava P;Lai HH;Mickle AD
通讯作者：
Mickle AD