Droplet Microfluidics with MALDI-MS Detection: The Effects of Oil Phases in GABA Analysis.
Droplet Microfluidics with MALDI-MS Detection: The Effects of Oil Phases in GABA Analysis.
复制标题
液滴微流控与MALDI-MS检测：GABA分析中油相的影响。
DOI：
10.1021/acsmeasuresciau.1c00017
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发表时间：
2021-12-15
期刊：
影响因子：
--
通讯作者：
Sweedler JV
中科院分区：
文献类型：
--
作者：
Bell SE;Park I;Rubakhin SS;Bashir R;Vlasov Y;Sweedler JV
微流体和质谱（MS）方法被广泛用于采样和探测生物系统的化学组成，以阐明其健康和疾病状态的化学相关性。虽然基质辅助激光解吸/电离质谱（MALDI）-MS已联用液滴微流体离线分析，与液滴生成相关的参数的影响，如所使用的油相的类型，已经研究不足。为了表征这些效果，在液滴微流体中测试了五种不同的油相，以产生用于MALDI-MS分析的样品。在聚二甲基硅氧烷微流体芯片内产生含有0.1至100 mM γ-氨基丁酸（GABA）和无机盐的皮升至纳升水性液滴，并沉积在导电载玻片上。光学显微镜，拉曼光谱，和MALDI质谱成像（MSI）的液滴样品和周围地区揭示了溶剂和油蒸发和分析物沉积的模式。光学显微镜检测到直径为50-100 μm的干燥液滴中存在盐晶体，并使用拉曼和MSI将GABA信号与可见液滴足迹相关联。MALDI-MS分析显示，在辛醇油存在下制备的液滴导致GABA的可检测性最差，而含有FC-40的油相提供了最好的可检测性; GABA信号定位于65 μ L液滴的足迹，检测限为23阿莫尔。还研究了表面活性剂全氟辛醇对分析物检测的影响。
Microfluidic and mass spectrometry (MS) methods are widely used to sample and probe the chemical composition of biological systems to elucidate chemical correlates of their healthy and disease states. Though matrix-assisted laser desorption/ionization-mass spectrometry (MALDI)-MS has been hyphenated to droplet microfluidics for offline analyses, the effects of parameters related to droplet generation, such as the type of oil phase used, have been understudied. To characterize these effects, five different oil phases were tested in droplet microfluidics for producing samples for MALDI-MS analysis. Picoliter to nanoliter aqueous droplets containing 0.1 to 100 mM γ-aminobutyric acid (GABA) and inorganic salts were generated inside a polydimethylsiloxane microfluidic chip and deposited onto a conductive glass slide. Optical microscopy, Raman spectroscopy, and MALDI-mass spectrometry imaging (MSI) of the droplet samples and surrounding areas revealed patterns of solvent and oil evaporation and analyte deposition. Optical microscopy detected the presence of salt crystals in 50–100 μm diameter dried droplets, and Raman and MSI were used to correlate GABA signals to the visible droplet footprints. MALDI-MS analyses revealed that droplets prepared in the presence of octanol oil led to the poorest detectability of GABA, whereas the oil phases containing FC-40 provided the best detectability; GABA signal was localized to the footprint of 65 pL droplets with a limit of detection of 23 amol. The effect of the surfactant perfluorooctanol on analyte detection was also investigated.
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影响因子：
4.6
作者：
Mesbah K;Thai R;Bregant S;Malloggi F
通讯作者：
Malloggi F
影响因子：
6.1
作者：
Moon, Hyejin;Wheeler, Aaron R.;Kim, Chang-Jin CJ''
通讯作者：
Kim, Chang-Jin CJ''
DOI：
10.1002/cphc.201701364
发表时间：
2018-05-22
期刊：
Chemphyschem : a European journal of chemical physics and physical chemistry
影响因子：
--
作者：
Do TD;Ellis JF;Neumann EK;Comi TJ;Tillmaand EG;Lenhart AE;Rubakhin SS;Sweedler JV
通讯作者：
Sweedler JV
影响因子：
7.4
作者：
Ekström, S;Ericsson, D;Laurell, T
通讯作者：
Laurell, T
影响因子：
7.4
作者：
Baluya, Dodge L.;Garrett, Timothy J.;Yost, Richard A.
通讯作者：
Yost, Richard A.