Optically Guided Single Cell Mass Spectrometry of Rat Dorsal Root Ganglia to Profile Lipids, Peptides and Proteins.
Optically Guided Single Cell Mass Spectrometry of Rat Dorsal Root Ganglia to Profile Lipids, Peptides and Proteins.
复制标题
DOI：
10.1002/cphc.201701364
复制
发表时间：
2018-05-22
期刊：
影响因子：
--
通讯作者：
Sweedler JV
中科院分区：
文献类型：
--
作者：
Do TD;Ellis JF;Neumann EK;Comi TJ;Tillmaand EG;Lenhart AE;Rubakhin SS;Sweedler JV
哺乳动物背根神经节（DRG）位于脊神经的背根上，含有初级感觉神经元的胞体。DRG细胞已根据其大小、形态、细胞内标志物、对刺激的反应和神经肽分为亚群。为了了解DRG化学异质性和细胞功能之间的联系，我们使用连续基质辅助激光解吸/电离质谱（MS）进行了光学引导的高通量单细胞分析，以检测单个DRG细胞中的脂质，肽和几种蛋白质。所得质谱的统计分析允许根据细胞形态和推测的主要细胞类型对DRG群体进行分层。一个小细胞亚群含有髓鞘蛋白，这是丰富的雪旺细胞，和质谱的几个较大的细胞含有峰匹配的神经丝，波形蛋白，髓鞘碱性蛋白S，和胸腺素β蛋白。在分析的1,000多个细胞中，大约78%产生了推定的富含肽的光谱，使该群体被分为三种不同的细胞类型。在一种细胞类型中仅观察到两个m/z 4404和5487的信号，但不能与我们先前的LC-MS分析结果相匹配。生命之谜：连续基质辅助激光解吸/电离质谱分析揭示了1,000多个大鼠背根神经节细胞的化学异质性。形态学上相似的细胞产生不同的质谱图，因此被分类为不同的细胞类型，并对细胞功能产生影响。一组罕见的细胞含有以前未检测到的肽的特点。
The mammalian dorsal root ganglia (DRG) are located on the dorsal roots of the spinal nerves and contain cell bodies of primary sensory neurons. DRG cells have been classified into subpopulations based on their size, morphology, intracellular markers, response to stimuli, and neuropeptides. To understand the connections between DRG chemical heterogeneity and cellular function, we performed optically guided, high-throughput single cell profiling using sequential matrix-assisted laser desorption/ionization mass spectrometry (MS) to detect lipids, peptides, and several proteins in individual DRG cells. Statistical analysis of the resulting mass spectra allows stratification of the DRG population according to cellular morphology and, presumably, major cell types. A subpopulation of small cells contained myelin proteins, which are abundant in Schwann cells, and mass spectra of several larger cells contained peaks matching neurofilament, vimentin, myelin basic protein S, and thymosin beta proteins. Of the over 1,000 cells analyzed, approximately 78% produced putative peptide-rich spectra, allowing the population to be classified into three distinct cell types. Two signals with m/z 4404 and 5487 were exclusively observed in a cell type, but could not be matched to results of our previous LC-MS analyses. Puzzle of Life: Chemical heterogeneity in more than 1,000 individual rat dorsal root ganglion cells is revealed by sequential matrix-assisted laser desorption/ionization mass spectrometry analyses. Morphologically similar cells yield distinct mass spectral profiles, and so are categorized into distinct cell types, with implications on cell function. An uncommon group of cells containing previously undetected peptides was characterized.
登录
查看更多内容
影响因子：
7.4
作者：
Do TD;Comi TJ;Dunham SJ;Rubakhin SS;Sweedler JV
通讯作者：
Sweedler JV
DOI：
10.1073/pnas.85.12.4530
发表时间：
1988-06-01
影响因子：
11.1
作者：
HOFFMAN, PN;CLEVELAND, DW
通讯作者：
CLEVELAND, DW
影响因子：
48
作者：
Giesen, Charlotte;Wang, Hao A. O.;Bodenmiller, Bernd
通讯作者：
Bodenmiller, Bernd
DOI：
10.1016/j.jasms.2006.02.012
发表时间：
2006-06-01
影响因子：
3.2
作者：
Keller, Bernd O.;Li, Liang
通讯作者：
Li, Liang
影响因子：
5
作者：
Kaya, Ibrahim;Brinet, Dimitri;Hanrieder, Jorg
通讯作者：
Hanrieder, Jorg