Involvement of orexin neurons in fasting- and central adenosine-induced hypothermia.
Involvement of orexin neurons in fasting- and central adenosine-induced hypothermia.
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DOI：
10.1038/s41598-018-21252-w
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发表时间：
2018-02-09
影响因子：
4.6
通讯作者：
Kuwaki T
中科院分区：
文献类型：
--
作者：
Futatsuki T;Yamashita A;Ikbar KN;Yamanaka A;Arita K;Kakihana Y;Kuwaki T
我们研究了食欲素神经元是否可能对禁食和腺苷诱导的低温起保护作用。我们首先在禁食24小时期间测量食欲素神经元消融（ORX-AB）小鼠和野生型（WT）对照小鼠的体温（BT）。正如预期的那样，在ORX-AB小鼠中BT下降的幅度和遭受低体温的时间长度大于WT小鼠。食欲素神经元是活跃的，就在发病前的低温和恢复期间所揭示的钙成像在体内使用G-CaMP。接下来，我们通过脑室内给予腺苷A1受体激动剂N6-环己基腺苷（CHA）来研究腺苷诱导的体温过低，该激动剂在ORX-AB和WT小鼠中均诱导体温过低。在ORX-AB小鼠中引发低温反应所需的CHA剂量比WT小鼠的剂量大10倍以上。一旦建立低温，ORX-AB小鼠的恢复似乎较慢。在恢复阶段的食欲素神经元的激活证实了c-Fos的免疫组织化学。我们建议，食欲素神经元发挥双重作用（增强剂在诱导阶段和补偿在恢复阶段）在腺苷诱导的低温和保护/补偿作用，在禁食诱导的低温。
We examined whether orexin neurons might play a protective role against fasting- and adenosine-induced hypothermia. We first measured body temperature (BT) in orexin neuron-ablated (ORX-AB) mice and wild-type (WT) controls during 24 hours of fasting. As expected, the magnitude of BT drop and the length of time suffering from hypothermia were greater in ORX-AB mice than in WT mice. Orexin neurons were active just before onset of hypothermia and during the recovery period as revealed by calcium imaging in vivo using G-CaMP. We next examined adenosine-induced hypothermia via an intracerebroventricular administration of an adenosine A1 receptor agonist, N6-cyclohexyladenosine (CHA), which induced hypothermia in both ORX-AB and WT mice. The dose of CHA required to initiate a hypothermic response in ORX-AB mice was more than 10 times larger than the dose for WT mice. Once hypothermia was established, the recovery was seemingly slower in ORX-AB mice. Activation of orexin neurons during the recovery phase was confirmed by immunohistochemistry for c-Fos. We propose that orexin neurons play dual roles (enhancer in the induction phase and compensator during the recovery phase) in adenosine-induced hypothermia and a protective/compensatory role in fasting-induced hypothermia.
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影响因子：
4.6
作者：
Inutsuka A;Yamashita A;Chowdhury S;Nakai J;Ohkura M;Taguchi T;Yamanaka A
通讯作者：
Yamanaka A
影响因子：
4.8
作者：
Pereira-da-Silva, M;Torsoni, M;Velloso, L
通讯作者：
Velloso, L
DOI：
10.1086/physzool.52.2.30152564
发表时间：
1979-01-01
期刊：
PHYSIOLOGICAL ZOOLOGY
影响因子：
--
作者：
HUDSON, JW;SCOTT, IM
通讯作者：
SCOTT, IM
影响因子：
5.3
作者：
Nakamura, K;Matsumura, K;Kaneko, T
通讯作者：
Kaneko, T
影响因子：
4.7
作者：
Carlin JL;Jain S;Gizewski E;Wan TC;Tosh DK;Xiao C;Auchampach JA;Jacobson KA;Gavrilova O;Reitman ML
通讯作者：
Reitman ML