Drosophila glucome screening identifies Ck1alpha as a regulator of mammalian glucose metabolism.
Drosophila glucome screening identifies Ck1alpha as a regulator of mammalian glucose metabolism.
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DOI：
10.1038/ncomms8102
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发表时间：
2015-05-21
影响因子：
16.6
通讯作者：
Graff, Jonathan M.
中科院分区：
文献类型：
--
作者：
Ugrankar, Rupali;Berglund, Eric;Akdemir, Fatih;Tran, Christopher;Kim, Min Soo;Noh, Jungsik;Schneider, Rebekka;Ebert, Benjamin;Graff, Jonathan M.
循环碳水化合物是一种基本的能量来源，其中的扰动是各种疾病的特征，糖尿病是最普遍的。然而，糖尿病及其特征性高血糖症的许多潜在基因仍然难以捉摸。在这里，我们使用生理和遗传审讯D。黑腹果蝇的基因组，以揭示'glucome'，一套完整的基因参与葡萄糖调节苍蝇。对1,000个基因的部分基因组筛选产生了10160个高脂血症“苍蝇”候选者，我们使用脂肪体和肌肉特异性敲除和生化测定对其进行分类。结果突出了小葡萄糖部分作为果蝇代谢的生理指标。在我们的筛选中发现的命中可能在哺乳动物葡萄糖稳态中具有保守的功能，因为在小鼠脂肪谱系中Ck 1 α的杂合和纯合突变体发展为糖尿病。我们的研究结果表明，葡萄糖在苍蝇生物学中发挥作用，在苍蝇中进行的遗传筛查可能会增加我们对哺乳动物病理生理学的理解。 糖尿病与葡萄糖代谢异常有关。在这里，作者在果蝇中进行了基因组筛选，以确定新的果蝇葡萄糖代谢调节因子，并表明在脂肪谱系中缺乏蛋白激酶CK 1 α的小鼠同源物的小鼠会患糖尿病。
Circulating carbohydrates are an essential energy source, perturbations in which are pathognomonic of various diseases, diabetes being the most prevalent. Yet many of the genes underlying diabetes and its characteristic hyperglycaemia remain elusive. Here we use physiological and genetic interrogations in D. melanogaster to uncover the ‘glucome', the complete set of genes involved in glucose regulation in flies. Partial genomic screens of ∼1,000 genes yield ∼160 hyperglycaemia ‘flyabetes' candidates that we classify using fat body- and muscle-specific knockdown and biochemical assays. The results highlight the minor glucose fraction as a physiological indicator of metabolism in Drosophila. The hits uncovered in our screen may have conserved functions in mammalian glucose homeostasis, as heterozygous and homozygous mutants of Ck1alpha in the murine adipose lineage, develop diabetes. Our findings demonstrate that glucose has a role in fly biology and that genetic screenings carried out in flies may increase our understanding of mammalian pathophysiology. Diabetes is associated with aberrations in glucose metabolism. Here the authors perform a genomic screen in fruit flies to identify new regulators of fly glucose metabolism, and show that mice lacking the murine homologue of one of their hits, the protein kinase CK1alpha, in the adipose lineage develop diabetes.
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影响因子：
--
作者：
MORRIS, JA;GARDNER, MJ
通讯作者：
GARDNER, MJ
DOI：
10.1016/j.bbrc.2008.06.104
发表时间：
2008-09-12
影响因子：
3.1
作者：
Breen, Danna M.;Sanli, Toran;Tsiani, Evangelia
通讯作者：
Tsiani, Evangelia
影响因子：
3.2
作者：
Deshpande, Anjali D.;Harris-Hayes, Marcie;Schootman, Mario
通讯作者：
Schootman, Mario
影响因子：
3.7
作者：
Broughton S;Alic N;Slack C;Bass T;Ikeya T;Vinti G;Tommasi AM;Driege Y;Hafen E;Partridge L
通讯作者：
Partridge L
影响因子：
--
作者：
BARON, AD;BRECHTEL, G;EDELMAN, SV
通讯作者：
EDELMAN, SV