Fibrinogen adsorption to biomaterials.
Fibrinogen adsorption to biomaterials.
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DOI：
10.1002/jbm.a.36460
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发表时间：
2018-10
期刊：
影响因子：
--
通讯作者：
Horbett TA
中科院分区：
文献类型：
--
作者：
Horbett TA
纤维蛋白原（Fibrinogen, Fg）吸附是细胞与生物材料粘附的重要机制，也是作者研究的重点。本文综述了我们在Fg吸附方面的工作，并适当地引用了相关的研究成果。Fg促进吸附和细胞粘附的分子特性将被描述。此外，Fg从缓冲液、与其他蛋白质的二元溶液和血浆中的吸附行为将被讨论，包括Vroman效应。本文将对血小板粘附于选择性缺乏Fg、玻璃体连接蛋白（Vn）、纤维连接蛋白（Fn）或血管性血友病因子（vWf）的血浆表面的研究进行综述。这些研究清楚地表明，在静态条件下，Fg在血小板粘附中起主要作用，Fg和vWf在流动悬浮液的粘附中都起主要作用，但Vn或Fn没有显著作用。然而，也有研究表明，血小板粘附与吸附Fg的总量相关性不强，但与Fg细胞结合域特异性抗体的结合相关性很强。简要概述了防止Fg吸附的无污表面。更广泛地讨论了Fg吸附后的结构变化，包括用物理化学和生物方法检测到的变化。本文还简要讨论了用计算方法测定吸附蛋白结构的最新进展。最后一节确定Fg吸附是决定植入物在软组织和血液中的生物相容性的最重要的单一事件。
Fibrinogen (Fg) adsorption is an important mechanism underlying cell adhesion to biomaterials and was the major focus of the author’s research career. This article summarizes our work on Fg adsorption, with citations of related work as appropriate. The molecular properties of Fg that promote adsorption and cell adhesion will be described. In addition, the adsorption behavior of Fg from buffer, binary solutions with other proteins, and blood plasma will be discussed, including the Vroman effect. Studies of platelet adhesion to surfaces preadsorbed with blood plasmas selectively deficient in Fg, vitronectin (Vn), fibronectin (Fn), or von Willebrand’s factor (vWf) will be reviewed. These studies clearly showed a major role for Fg in platelet adhesion under static conditions and both Fg and vWf for adhesion from flowing suspensions, but no significant role for Vn or Fn. However, it was also shown that platelet adhesion was poorly correlated with the total amount of adsorbed Fg, but very well correlated with the binding of antibodies specific to the cell binding domains of Fg. A brief overview of nonfouling surfaces for prevention of Fg adsorption will be given. A more extensive discussion of structural changes in Fg after its adsorption is included, including changes detected with both physicochemical and biological methods. A short discussion of the state of the art of structural determination of adsorbed proteins with computational methods is also given. A final section identifies Fg adsorption as the single most important event determining the biocompatibility of implants in soft tissue and in blood.
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DOI：
10.1002/jbm.820210808
发表时间：
1987-08-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
ELWING, H;ASKENDAL, A;LUNDSTROM, I
通讯作者：
LUNDSTROM, I
影响因子：
14
作者：
CHINN, JA;RATNER, BD;HORBETT, TA
通讯作者：
HORBETT, TA
影响因子：
2.1
作者：
Abramyan, Tigran M.;Hyde-Volpe, David L.;Latour, Robert A.
通讯作者：
Latour, Robert A.
影响因子：
4.9
作者：
Cao, Lan;Chang, Mark;Horbett, Thomas A.
通讯作者：
Horbett, Thomas A.
DOI：
10.1083/jcb.5.1.11
发表时间：
1959-01-01
期刊：
JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY
影响因子：
--
作者：
HALL, CE;SLAYTER, HS
通讯作者：
SLAYTER, HS