Campylobacter jejuni survival within human epithelial cells is enhanced by the secreted protein CiaI.
Campylobacter jejuni survival within human epithelial cells is enhanced by the secreted protein CiaI.
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DOI：
10.1111/j.1365-2958.2011.07645.x
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发表时间：
2011-06
影响因子：
3.6
通讯作者：
Konkel ME
中科院分区：
文献类型：
--
作者：
Buelow DR;Christensen JE;Neal-McKinney JM;Konkel ME
尽管众所周知空肠弯曲杆菌会侵入人类肠道内的细胞，但使这种病原体能够在含有弯曲杆菌的液泡（CCV）内生存的细菌蛋白尚未确定。在这里，我们描述了一种蛋白质的鉴定和表征，我们将其命名为 CiaI，用于与细胞内存活相关的弯曲杆菌入侵抗原。我们发现 CiaI 具有氨基末端 III 型分泌 (T3S) 序列，并通过鞭毛 T3S 系统从空肠弯曲菌分泌。此外，根据庆大霉素保护试验判断，与野生型菌株相比，ciaI突变体的细胞内存活受到损害。对感染空肠弯曲菌ciaI突变体的上皮细胞进行荧光显微镜检查发现，与感染空肠弯曲菌野生型菌株的细胞中的CCV相比，CCV更频繁地与组织蛋白酶D（溶酶体标记物）共定位。上皮细胞中 CiaI-GFP 的异位表达产生了其他空肠弯曲菌基因未观察到的点状表型，并且该表型因位于蛋白质羧基末端的双亮氨酸基序的突变而被消除。根据数据，我们得出结论，CiaI 有助于空肠弯曲菌在上皮细胞内存活的能力。
Although it is known that Campylobacter jejuni invade the cells that line the human intestinal tract, the bacterial proteins that enable this pathogen to survive within Campylobacter-containing vacuoles (CCV) have not been identified. Here, we describe the identification and characterization of a protein that we termed CiaI for Campylobacter invasion antigen involved in Intracellular survival. We show that CiaI harbors an amino-terminal type III secretion (T3S) sequence and is secreted from C. jejuni through the flagellar T3S system. In addition, the ciaI mutant was impaired in intracellular survival when compared to a wild-type strain, as judged by the gentamicin-protection assay. Fluorescence microscopy examination of epithelial cells infected with the C. jejuni ciaI mutant revealed that the CCV were more frequently co-localized with Cathepsin D (a lysosomal marker) than the CCV in cells infected with a C. jejuni wild-type strain. Ectopic expression of CiaI-GFP in epithelial cells yielded a punctate phenotype not observed with the other C. jejuni genes, and this phenotype was abolished by mutation of a dileucine motif located in the carboxy-terminus of the protein. Based on the data, we conclude that CiaI contributes to the ability of C. jejuni to survive within epithelial cells.
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影响因子：
11.8
作者：
Coker AO;Isokpehi RD;Thomas BN;Amisu KO;Obi CL
通讯作者：
Obi CL
影响因子：
3.6
作者：
Christensen JE;Pacheco SA;Konkel ME
通讯作者：
Konkel ME
影响因子：
3.6
作者：
Konkel, ME;Kim, BJ;Garvis, SG
通讯作者：
Garvis, SG
影响因子：
3.2
作者：
Konkel, ME;Klena, JD;Mickelson, J
通讯作者：
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64.8
作者：
Kelly, Bernard T.;McCoy, Airlie J.;Spaete, Kira;Miller, Sharon E.;Evans, Philip R.;Hoening, Stefan;Owen, David J.
通讯作者：
Owen, David J.