Identification of genes required for Drosophila eye development using a phenotypic enhancer-trap.
Identification of genes required for Drosophila eye development using a phenotypic enhancer-trap.
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使用表型增强子陷阱鉴定果蝇眼睛发育所需的基因。
DOI：
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发表时间：
1997
影响因子：
11.1
通讯作者：
Zipursky Sl
中科院分区：
文献类型：
--
作者：
F. Pignoni;B. Hu;Zipursky Sl
描述了一种新的p元素诱变方法，用于分离影响果蝇复眼发育的突变体。它利用了seven - less （Boss）配体和seven - less （Sev）受体酪氨酸激酶之间的相互作用，从而触发了紫外线敏感感光神经元R7的形成。在其他boss突变背景下，将boss cDNA转基因转位作为活蝇的“表型陷阱”，以鉴定在眼睛发育的窄时间窗内表达的增强子。使用快速行为筛选，对40多万只苍蝇进行了R7恢复测试。鉴定出约1800只含有r7的苍蝇。其中，通过眼外形态学和boss抗体染色，在R8细胞中鉴定了21个表达boss报告基因的独立插入。在900个在多个细胞中表达boss报告基因的细胞系中，分离出了marbles、glass、gap1和fasciclin II基因的插入。这种表型增强子陷阱有助于(i)分离具有特定表达模式的增强子陷阱，以及（ii）恢复破坏特定组织发育的突变体。由于表型陷阱的时间和组织特异性取决于所使用的标记的选择，因此这种方法可以扩展到其他组织和发育阶段。
A novel method of P-element mutagenesis is described for the isolation of mutants affecting the development of the Drosophila compound eye. It exploits the interaction between the Bride of Sevenless (Boss) ligand and the Sevenless (Sev) receptor tyrosine kinase that triggers the formation of the UV-sensitive photoreceptor neuron, R7. Transposition of a boss cDNA transgene, in an otherwise boss mutant background, was used as a "phenotypic trap" in live flies to identify enhancers expressed during a narrow time window in eye development. Using a rapid behavioral screen, more than 400,000 flies were tested for restoration of R7. Some 1,800 R7-containing flies were identified. Among these, 21 independent insertions with expression of the boss reporter gene in the R8 cell were identified by a external eye morphology and staining with an antibody against Boss. Among 900 lines with expression of the boss reporter gene in multiple cells assessed for homozygous mutant phenotypes, insertions in the marbles, glass, gap1, and fasciclin II genes were isolated. This phenotypic enhancer-trap facilitates (i) the isolation of enhancer-traps with a specific expression pattern, and (ii) the recovery of mutants disrupting development of specific tissues. Because the temporal and tissue specificity of the phenotypic trap is dependent on the choice of the marker used, this approach can be extended to other tissues and developmental stages.
DOI：
10.1073/pnas.92.23.10501
发表时间：
1995-11-07
影响因子：
11.1
作者：
GARCIAALONSO, L;VANBERKUM, MFA;GOODMAN, CS
通讯作者：
GOODMAN, CS
影响因子：
10.5
作者：
HART, AC;KRAMER, H;ZIPURSKY, SL
通讯作者：
ZIPURSKY, SL
大理石突变体：果蝇眼睛发育中的细胞测定和核迁移的解偶联。
DOI：
10.1242/dev.120.9.2609
发表时间：
1994
期刊：
Development (Cambridge, England)
影响因子：
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作者：
Fischer-Vize,JA;Mosley,KL
通讯作者：
Mosley,KL