DMSO triggers the generation of ROS leading to an increase in artemisinin and dihydroartemisinic acid in Artemisia annua shoot cultures.
DMSO triggers the generation of ROS leading to an increase in artemisinin and dihydroartemisinic acid in Artemisia annua shoot cultures.
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DOI：
10.1007/s00299-009-0807-y
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发表时间：
2010-02
影响因子：
6.2
通讯作者：
Weathers, Pamela J.
中科院分区：
文献类型：
--
作者：
Mannan, Abdul;Liu, Chunzhao;Arsenault, Patrick R.;Towler, Melissa J.;Vail, Dan R.;Lorence, Argelia;Weathers, Pamela J.
抗疟疾倍半萜烯青蒿素供应短缺；需求没有得到满足，青蒿素在植物中的作用尚未明确。先前的研究表明，向幼苗中添加二甲亚砜 (DMSO) 会增加芽中青蒿素的含量，本研究进一步研究了这一偶然的观察结果。当向体外培养的青蒿根芽添加不同量的 DMSO (0-2.0% v/v) 时，青蒿素水平加倍，并在 0.25 和 2.0% DMSO 时显示出双相最佳值。青蒿素及其前体二氢青蒿酸均增加，其中前者在 DMSO 处理后持续 7 天。经 DMSO 处理的未根芽没有刺激青蒿素的产生。青蒿素生物合成途径中的第一个基因紫穗槐二烯合酶与对照相比，在 DMSO 的作用下转录水平没有增加。相比之下，该途径中的第二个基因 CYP71AV1 确实对 DMSO 有反应，但转录物水平与青蒿素水平相反。当生根芽进行活性氧（ROS）、H2O2染色时，ROS随着DMSO浓度的增加而增加；无根芽不产生 DMSO 反应的 ROS。添加维生素 C 可以抑制 DMSO 诱导的 ROS 反应和相应的青蒿素水平的增加。这些数据共同表明，至少在对 DMSO 的反应中，青蒿素的产量和 ROS 增加，并且当 ROS 减少时，青蒿素也减少，这表明 ROS 可能在青蒿的青蒿素生产中发挥作用。
The antimalarial sesquiterpene, artemisinin, is in short supply; demand is not being met, and the role of artemisinin in the plant is not well established. Prior work showed that addition of dimethyl sulfoxide (DMSO) to seedlings increased artemisinin in their shoots and this study further investigated that serendipitous observation. When in vitro-cultured Artemisia annua rooted shoots were fed different amounts of DMSO (0–2.0% v/v), artemisinin levels doubled and showed biphasic optima at 0.25 and 2.0% DMSO. Both artemisinin and its precursor, dihydroartemisinic acid, increased with the former continuing 7 days after DMSO treatment. There was no stimulation of artemisinin production in DMSO-treated unrooted shoots. The first gene in the artemisinin biosynthetic pathway, amorphadiene synthase, showed no increase in transcript level in response to DMSO compared to controls. In contrast, the second gene in the pathway, CYP71AV1, did respond to DMSO but at a level of transcripts inverse to artemisinin levels. When rooted shoots were stained for the reactive oxygen species (ROS), H2O2, ROS increased with increasing DMSO concentration; unrooted shoots produced no ROS in response to DMSO. Both the increases in DMSO-induced ROS response and corresponding artemisinin levels were inhibited by addition of vitamin C. Together these data show that at least in response to DMSO, artemisinin production and ROS increase and that when ROS is reduced, so also is artemisinin suggesting that ROS may play a role in artemisinin production in A. annua.
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影响因子：
7.6
作者：
Romero, MR;Efferth, T;Marin, JJG
通讯作者：
Marin, JJG
影响因子：
2.1
作者：
Brown, GD;Sy, LK
通讯作者：
Sy, LK
影响因子：
2.1
作者：
Brown, Geoffrey D.;Sy, Lai-King
通讯作者：
Sy, Lai-King
DOI：
10.1111/j.1749-6632.1983.tb47334.x
发表时间：
1983-01-01
影响因子：
5.2
作者：
KHARASCH, N;THYAGARAJAN, BS
通讯作者：
THYAGARAJAN, BS
影响因子：
2.7
作者：
Hernandez, Xenia E;Orden, Alejandro A;Kurina, Marcela
通讯作者：
Kurina, Marcela