Rubiscosome gene expression is balanced across the hexaploid wheat genome.
Rubiscosome gene expression is balanced across the hexaploid wheat genome.
复制标题
DOI：
10.1007/s11120-022-00897-9
复制
发表时间：
2022-04
影响因子：
3.7
通讯作者：
Carmo-Silva, Elizabete
中科院分区：
文献类型：
--
作者：
Caruana, Louis;Orr, Douglas J.;Carmo-Silva, Elizabete
功能性和活性Rubisco对于CO2固定是必不可少的，并且是提高作物产量的工程方法的主要目标。然而，活性Rubisco的组装和维持依赖于至少11种核编码蛋白的协调生物合成，称为“Rubiscosome”。使用公开可用的小麦（Triticum aestivum L.）基因表达数据，我们表明，在形成异源六倍体基因组的三个密切相关的亚基因组中，Rubiscosome基因的表达是平衡的。每个亚基因组包含一个接近完整的同源异型基因集，并在最佳和热应激条件下对整体表达做出同等贡献。小麦耐热Rubisco活化酶同工型1β的表达在热胁迫下增加，并且在亚基因组之间保持平衡，尽管D亚基因组的贡献略有增加。研究结果表明，在设计作物改良策略时，需要考虑所有三个亚基因组中的基因拷贝。在线版本包含补充材料，可通过10.1007/s11120-022-00897-9获得。
Functional and active Rubisco is essential for CO2 fixation and is a primary target for engineering approaches to increasing crop yields. However, the assembly and maintenance of active Rubisco are dependent on the coordinated biosynthesis of at least 11 nuclear-encoded proteins, termed the ‘Rubiscosome’. Using publicly available gene expression data for wheat (Triticum aestivum L.), we show that the expression of Rubiscosome genes is balanced across the three closely related subgenomes that form the allohexaploid genome. Each subgenome contains a near complete set of homoeologous genes and contributes equally to overall expression, both under optimal and under heat stress conditions. The expression of the wheat thermo-tolerant Rubisco activase isoform 1β increases under heat stress and remains balanced across the subgenomes, albeit with a slight shift towards greater contribution from the D subgenome. The findings show that the gene copies in all three subgenomes need to be accounted for when designing strategies for crop improvement. The online version contains supplementary material available at 10.1007/s11120-022-00897-9.
登录
查看更多内容
影响因子：
5.3
作者：
Liu Z;Xin M;Qin J;Peng H;Ni Z;Yao Y;Sun Q
通讯作者：
Sun Q
影响因子：
12.3
作者：
Krasileva KV;Buffalo V;Bailey P;Pearce S;Ayling S;Tabbita F;Soria M;Wang S;IWGS Consortium;Akhunov E;Uauy C;Dubcovsky J
通讯作者：
Dubcovsky J
影响因子：
7.3
作者：
Conlan, Brendon;Birch, Rosemary;Whitney, Spencer M.
通讯作者：
Whitney, Spencer M.
影响因子：
7.2
作者：
Degen, Gustaf E.;Worrall, Dawn;Carmo-Silva, Elizabete
通讯作者：
Carmo-Silva, Elizabete
影响因子：
5
作者：
Vitlin Gruber A;Feiz L
通讯作者：
Feiz L