Temporal transcriptome profiling reveals expression partitioning of homeologous genes contributing to heat and drought acclimation in wheat (Triticum aestivum L.).
Temporal transcriptome profiling reveals expression partitioning of homeologous genes contributing to heat and drought acclimation in wheat (Triticum aestivum L.).
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DOI：
10.1186/s12870-015-0511-8
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发表时间：
2015-06-20
影响因子：
5.3
通讯作者：
Sun Q
中科院分区：
文献类型：
--
作者：
Liu Z;Xin M;Qin J;Peng H;Ni Z;Yao Y;Sun Q
六倍体小麦(Triticum Aestivum)是一种全球重要作物。高温、干旱及其组合极大地降低了小麦的产量和品质，但小麦对极端环境，特别是胁迫组合耐性的分子机制在很大程度上还不清楚。作为异源六倍体，小麦由三个紧密相关的亚基因组(A、B和D)组成，据报道，与四倍体相比，小麦表现出更强的抗逆性。但到目前为止，人们对小麦如何协调同源基因的表达以应对全基因组水平上的各种环境限制知之甚少。为了探索小麦对单独胁迫和复合胁迫的转录响应，我们对正常条件下以及干旱胁迫(DS)、高温胁迫(HS)及其组合(HD)胁迫1h和6h的小麦幼苗进行了高通量转录组测序，并提供了响应这三种胁迫的整体基因表达重组程序。对DS、HS和HD反应基因的基因本体论(GO)浓缩分析表明，它们之间的功能通路相互重叠和复杂。此外，根据IWGSC发布的支架信息，在全基因组水平上鉴定了4,375个小麦转录因子，其中1,328个对胁迫处理有反应。然后，HSFs和DREB的调控网络分析表明，它们都参与了DS、HS和HD反应的调控，并表明了高温和干旱胁迫之间的相互作用。最后，约68.4%的同源基因表现出对DS、HS或HD的响应，这一点通过定量RT-PCR和缺体-四体系进一步得到证实。大部分小麦同源基因在正常和非生物胁迫下表现出表达分配，这可能是六倍体小麦对各种环境约束具有广泛的适应性和分布的原因。本文的在线版本(doi：10.1186/s12870-0150511-8)包含补充材料，授权用户可以使用。
Hexaploid wheat (Triticum aestivum) is a globally important crop. Heat, drought and their combination dramatically reduce wheat yield and quality, but the molecular mechanisms underlying wheat tolerance to extreme environments, especially stress combination, are largely unknown. As an allohexaploid, wheat consists of three closely related subgenomes (A, B, and D), and was reported to show improved tolerance to stress conditions compared to tetraploid. But so far very little is known about how wheat coordinates the expression of homeologous genes to cope with various environmental constraints on the whole-genome level. To explore the transcriptional response of wheat to the individual and combined stress, we performed high-throughput transcriptome sequencing of seedlings under normal condition and subjected to drought stress (DS), heat stress (HS) and their combination (HD) for 1 h and 6 h, and presented global gene expression reprograms in response to these three stresses. Gene Ontology (GO) enrichment analysis of DS, HS and HD responsive genes revealed an overlap and complexity of functional pathways between each other. Moreover, 4,375 wheat transcription factors were identified on a whole-genome scale based on the released scaffold information by IWGSC, and 1,328 were responsive to stress treatments. Then, the regulatory network analysis of HSFs and DREBs implicated they were both involved in the regulation of DS, HS and HD response and indicated a cross-talk between heat and drought stress. Finally, approximately 68.4 % of homeologous genes were found to exhibit expression partitioning in response to DS, HS or HD, which was further confirmed by using quantitative RT-PCR and Nullisomic-Tetrasomic lines. A large proportion of wheat homeologs exhibited expression partitioning under normal and abiotic stresses, which possibly contributes to the wide adaptability and distribution of hexaploid wheat in response to various environmental constraints. The online version of this article (doi:10.1186/s12870-015-0511-8) contains supplementary material, which is available to authorized users.
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影响因子：
4.4
作者：
Aprile A;Mastrangelo AM;De Leonardis AM;Galiba G;Roncaglia E;Ferrari F;De Bellis L;Turchi L;Giuliano G;Cattivelli L
通讯作者：
Cattivelli L
影响因子：
5.6
作者：
Golldack D;Li C;Mohan H;Probst N
通讯作者：
Probst N
影响因子：
3.5
作者：
Dhanda, SS;Sethi, GS;Behl, RK
通讯作者：
Behl, RK
影响因子：
9.4
作者：
Dong, Shaowei;Adams, Keith L.
通讯作者：
Adams, Keith L.
影响因子：
3.3
作者：
Gill, Bikram S.;Appels, Rudi;Sasaki, Takuji
通讯作者：
Sasaki, Takuji