Long-Term High-Density Extracellular Recordings Enable Studies of Muscle Cell Physiology.
Long-Term High-Density Extracellular Recordings Enable Studies of Muscle Cell Physiology.
复制标题
DOI：
10.3389/fphys.2018.01424
复制
发表时间：
2018
影响因子：
4
通讯作者：
Punga AR
中科院分区：
文献类型：
--
作者：
Lewandowska MK;Bogatikov E;Hierlemann AR;Punga AR
骨骼肌（随意肌）是体内最丰富的组织，因此成为重要的生物医学研究课题。神经肌肉传递的研究，包括自身免疫性或遗传性神经肌肉疾病中的离子通道或受体疾病，需要高空间分辨率测量技术和随时间推移获取重复记录的能力，以便跟踪药理干预措施。通过组织-组织和细胞-细胞相互作用的生理离体模型可以增强研究神经肌肉传递疾病的临床前技术。在这里，我们提出了一种方法，可以在两个多月的时间内跟踪初级骨骼肌细胞从成肌细胞到成熟收缩肌管的发育。与之前的大多数研究相反，肌管并没有从表面分离，而是在肌管之间形成功能网络，在整个培养期间观察到肌管的电信号。小鼠成肌细胞的原代培养物在芯片上分化为收缩肌管，该芯片包含 26,400 个铂电极阵列，密度为每平方毫米 3,265 个电极。我们以亚细胞分辨率追踪细胞外动作电位的能力使得我们能够研究骨骼肌的发育和动力学、尖峰模式以及肌肉之间的时空关系。所开发的系统反过来又能够创建用于建立离体疾病模型的新型电生理学平台。
Skeletal (voluntary) muscle is the most abundant tissue in the body, thus making it an important biomedical research subject. Studies of neuromuscular transmission, including disorders of ion channels or receptors in autoimmune or genetic neuromuscular disorders, require high-spatial-resolution measurement techniques and an ability to acquire repeated recordings over time in order to track pharmacological interventions. Preclinical techniques for studying diseases of neuromuscular transmission can be enhanced by physiologic ex vivo models of tissue-tissue and cell-cell interactions. Here, we present a method, which allows tracking the development of primary skeletal muscle cells from myoblasts into mature contracting myotubes over more than 2 months. In contrast to most previous studies, the myotubes did not detach from the surface but instead formed functional networks between the myotubes, whose electrical signals were observed over the entire culturing period. Primary cultures of mouse myoblasts differentiated into contracting myotubes on a chip that contained an array of 26,400 platinum electrodes at a density of 3,265 electrodes per mm2. Our ability to track extracellular action potentials at subcellular resolution enabled study of skeletal muscle development and kinetics, modes of spiking and spatio-temporal relationships between muscles. The developed system in turn enables creation of a novel electrophysiological platform for establishing ex vivo disease models.
登录
查看更多内容
DOI：
10.1016/j.vascn.2012.04.001
发表时间：
2012-05
影响因子：
1.9
作者：
Grosberg A;Nesmith AP;Goss JA;Brigham MD;McCain ML;Parker KK
通讯作者：
Parker KK
影响因子：
5.5
作者：
Bandi, E;Bernareggi, A;Lorenzon, P
通讯作者：
Lorenzon, P
影响因子：
4.6
作者：
LEFEVER, RS;DE LUCA, CJ
通讯作者：
DE LUCA, CJ
影响因子：
4.5
作者：
JACKSON, MB;IMOTO, K;SAKMANN, B
通讯作者：
SAKMANN, B
影响因子：
5.4
作者：
Frey, Urs;Sedivy, Jan;Hierlemann, Andreas
通讯作者：
Hierlemann, Andreas