Effects of Extra Virgin Olive Oil Polyphenols on Beta-Cell Function and Survival.
Effects of Extra Virgin Olive Oil Polyphenols on Beta-Cell Function and Survival.
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DOI：
10.3390/plants10020286
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发表时间：
2021-02-03
期刊：
影响因子：
--
通讯作者：
Natalicchio A
中科院分区：
文献类型：
--
作者：
Marrano N;Spagnuolo R;Biondi G;Cignarelli A;Perrini S;Vincenti L;Laviola L;Giorgino F;Natalicchio A
特级初榨橄榄油（EVOO）是地中海饮食的主要组成部分，因其对代谢疾病（包括2型糖尿病（T2 D））的营养益处而受到全世界的赞赏。EVOO含有大量的次级代谢产物，如酚类化合物（PC），可能对代谢状态产生积极影响。在这项研究中，我们首次研究了几种PC对β细胞功能和存活的影响。为此，将INS-1 E细胞暴露于10 μM的主要EVOO PC长达24 h。在这些条件下，评估了存活率、胰岛素生物合成、葡萄糖刺激的胰岛素分泌（GSIS）和细胞内信号传导激活（蛋白激酶B（AKT）和cAMP反应元件结合蛋白（CREB））。羟基酪醇、酪醇和芹菜素增强β细胞增殖和胰岛素生物合成，芹菜素和毛地黄黄酮增强GSIS。相反，香草酸和香草醛是β细胞的促凋亡剂，即使它们增加了GSIS。此外，橄榄苦苷，p-香豆酸，阿魏酸和芥子酸显着恶化GSIS。最后，羟基酪醇、酪醇和芹菜素的混合物促进人胰岛中的GSIS。芹菜素是最有效的化合物，也能够激活有益的细胞内信号传导。总之，这项研究表明羟基酪醇、酪醇和芹菜素促进β细胞的健康，这表明富含这些化合物的EVOO或补充剂可以改善T2 D患者的胰岛素分泌并促进血糖控制。
Extra virgin olive oil (EVOO) is a major component of the Mediterranean diet and is appreciated worldwide because of its nutritional benefits in metabolic diseases, including type 2 diabetes (T2D). EVOO contains significant amounts of secondary metabolites, such as phenolic compounds (PCs), that may positively influence the metabolic status. In this study, we investigated for the first time the effects of several PCs on beta-cell function and survival. To this aim, INS-1E cells were exposed to 10 μM of the main EVOO PCs for up to 24 h. Under these conditions, survival, insulin biosynthesis, glucose-stimulated insulin secretion (GSIS), and intracellular signaling activation (protein kinase B (AKT) and cAMP response element-binding protein (CREB)) were evaluated. Hydroxytyrosol, tyrosol, and apigenin augmented beta-cell proliferation and insulin biosynthesis, and apigenin and luteolin enhanced the GSIS. Conversely, vanillic acid and vanillin were pro-apoptotic for beta-cells, even if they increased the GSIS. In addition, oleuropein, p-coumaric, ferulic and sinapic acids significantly worsened the GSIS. Finally, a mixture of hydroxytyrosol, tyrosol, and apigenin promoted the GSIS in human pancreatic islets. Apigenin was the most effective compound and was also able to activate beneficial intracellular signaling. In conclusion, this study shows that hydroxytyrosol, tyrosol, and apigenin foster beta-cells’ health, suggesting that EVOO or supplements enriched with these compounds may improve insulin secretion and promote glycemic control in T2D patients.
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纯橄榄油酚类化合物的健康受益机制。
DOI：
10.3390/molecules21121734
发表时间：
2016-12-16
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Parkinson L;Cicerale S
通讯作者：
Cicerale S
DOI：
10.1016/j.bbrc.2015.12.036
发表时间：
2016-01-15
影响因子：
3.1
作者：
Lee, Hyunjung;Im, Sung Won;Ahn, Jiyun
通讯作者：
Ahn, Jiyun
影响因子：
5.1
作者：
Cho, N. H.;Shaw, J. E.;Malanda, B.
通讯作者：
Malanda, B.
影响因子：
5.4
作者：
Santangelo, C.;Filesi, C.;Masella, R.
通讯作者：
Masella, R.
影响因子：
3.6
作者：
Suarez, Manuel;Valls, Rosa M.;Motilva, Maria-Jose
通讯作者：
Motilva, Maria-Jose