Identification of differentially expressed genes in rat aortic allograft vasculopathy.
Identification of differentially expressed genes in rat aortic allograft vasculopathy.
复制标题
大鼠同种异体主动脉血管病差异表达基因的鉴定。
作者：
J. Chen;M. Myllärniemi;L. Akyürek;P. Häyry;P. Marsden;L. Paul
移植物血管病变是长期存活器官移植的重要并发症，但其发病机制仍不明确。我们研究了有血管病变的大鼠主动脉移植、无血管病变的大鼠主动脉移植和正常主动脉的差异表达mRNA转录物，以进一步了解其中的分子机制。在同种异体或同基因受体中进行主动脉移植，1或5个月后切除，分离RNA，并鉴别显示mRNA转录物，其表达与移植过程和血管病变的发展相关。使用80个随机引物，鉴定出57个差异显示的聚合酶链反应产物，其中18个在同种异体移植物中发现，而在同基因移植物或正常血管中没有，而15个在正常血管和同基因移植物中表达，而在同种异体移植物中没有。在差异显示的扩增子中，有13个被成功地重新扩增，并用作Northern分析的探针；6例证实有差异表达。PCR产物的DNA序列分析显示，其中2例与免疫球蛋白J链、铁蛋白重链（与Ras相关但不完全相同的序列）和最近从人胎儿心脏文库中分离到的已建立的序列标签一致；1个序列与已知基因无亲缘关系。为了评估j链基因（一种在B淋巴细胞系细胞中表达的基因）的差异mRNA表达是否与B淋巴细胞浸润移植物有关，用B细胞标记物CD45RA抗体对组织切片进行染色。虽然cd45ra阳性细胞数量较少，但血管病变移植物的外膜和内膜中cd45ra阳性细胞数量明显增加。此外，用抗铁蛋白抗血清免疫染色证实了在移植物血管壁内层存在铁蛋白阳性细胞，并分散在内膜、中膜和外膜中。问题仍然是这些基因中哪些在移植物血管病变的发病机制中至关重要，以及它们是否可以作为治疗干预的靶点。
Graft vasculopathy is an important complication of long-surviving organ transplants, but its pathogenesis has remained elusive. We investigated rat aortic transplants with vasculopathy, aortic transplants without vasculopathy, and normal aortas for differentially expressed mRNA transcripts to gain further insight into the molecular mechanisms involved. Aortic transplants were performed in allogeneic or syngeneic recipients followed by removal after 1 or 5 months, RNA isolation, and differential display to identify mRNA transcripts the expression of which was modulated in conjunction with the transplant procedure and the development of vasculopathy. Using 80 random primers, 57 differentially displayed polymerase chain reaction products were identified, 18 of which were found in allografts but not in syngeneic grafts or normal vessels, whereas 15 were expressed in normal vessels and syngeneic grafts but not in allografts. Of the differentially displayed amplicons, 13 were successfully reamplified and used as probes for Northern analysis; differential expression was confirmed in 6 instances. DNA sequence analysis of these PCR products revealed identity with the immunoglobulin J chain in 2 instances, the ferritin heavy chain, a sequence related but not identical with Ras, and an established sequence tag recently isolated from a human fetal heart library; 1 sequence was not related to any known gene. To assess whether differential mRNA expression of the J-chain gene, a gene expressed in cells of B lymphocyte lineage, was associated with infiltration of the graft by B lymphocytes, tissue sections were stained with an antibody against the B cell marker CD45RA. Although the number of CD45RA-positive cells was low, there was a significant increase in the number of CD45RA-positive cells in the adventitia and intima of grafts with vasculopathy. Furthermore, immunostaining with anti-ferritin antiserum confirmed the presence of ferritin-positive cells within the inner layer of the graft vessel wall and dispersed in the intima, media, and adventitia. The question remains as to which of these genes are critically relevant in the pathogenesis of graft vasculopathy and whether they serve as targets for therapeutic interventions.
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分化 Friend 红白血病细胞中的铁蛋白合成。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Beaumont,C;Jain,SK;Bogard,M;Nordmann,Y;Drysdale,J
通讯作者：
Drysdale,J
IL-10 信使 RNA 的移植物内表达：肾同种异体移植排斥的新相关因素。
影响因子：
19.6
作者：
Xu,GP;Sharma,VK;Li,B;Bologa,R;Li,Y;Mouradian,J;Wang,J;Serur,D;Rao,V;Stenzel,KH
通讯作者：
Stenzel,KH
对白细胞介素 2 反应性 J 链基因发挥正向和负向控制的启动子元件。
DOI：
10.1073/pnas.89.13.5966
发表时间：
1992
影响因子：
11.1
作者：
Lansford,RD;McFadden,HJ;Siu,ST;Cox,JS;Cann,GM;Koshland,ME
通讯作者：
Koshland,ME
影响因子：
6.2
作者：
Cramer,DV;Chapman,FA;Wu,GD;Harnaha,JB;Qian,SQ;Makowka,L
通讯作者：
Makowka,L
影响因子：
14.9
作者：
LIANG, P;AVERBOUKH, L;PARDEE, AB
通讯作者：
PARDEE, AB