TRPV4 channel is involved in the coupling of fluid viscosity changes to epithelial ciliary activity.
TRPV4 channel is involved in the coupling of fluid viscosity changes to epithelial ciliary activity.
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DOI：
10.1083/jcb.200409070
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发表时间：
2005-03-14
期刊：
影响因子：
--
通讯作者：
Valverde MA
中科院分区：
文献类型：
--
作者：
Andrade YN;Fernandes J;Vázquez E;Fernández-Fernández JM;Arniges M;Sánchez TM;Villalón M;Valverde MA
纤毛搏动频率（CBF）的自动调节已被提出作为上皮纤毛细胞用于维持CBF并防止在不同粘液粘度的条件下粘液纤毛运输崩溃的机制。尽管这种调节反应与气道和生殖道的病理生理学相关，但其潜在的细胞和分子方面仍然未知。输卵管纤毛细胞表达瞬时受体电位香草酸4（TRPV 4）通道，其通过涉及磷脂酶A2依赖性途径的粘性负荷增加而被激活。TRPV 4转染的HeLa细胞也增加了它们的阳离子电流响应于高粘性负荷。这种机械活化在负载有TRPV 4抗体的天然纤毛细胞中被阻止。TRPV 4合成配体4α-佛波醇12，13-二癸酸酯的应用增加了阳离子电流、细胞内Ca 2+和在不存在粘性负荷的情况下的CBF。因此，TRPV 4成为参与流体粘度变化与CBF自动调节所需的Ca 2+信号的产生的耦合的候选者。
Autoregulation of the ciliary beat frequency (CBF) has been proposed as the mechanism used by epithelial ciliated cells to maintain the CBF and prevent the collapse of mucociliary transport under conditions of varying mucus viscosity. Despite the relevance of this regulatory response to the pathophysiology of airways and reproductive tract, the underlying cellular and molecular aspects remain unknown. Hamster oviductal ciliated cells express the transient receptor potential vanilloid 4 (TRPV4) channel, which is activated by increased viscous load involving a phospholipase A2–dependent pathway. TRPV4-transfected HeLa cells also increased their cationic currents in response to high viscous load. This mechanical activation is prevented in native ciliated cells loaded with a TRPV4 antibody. Application of the TRPV4 synthetic ligand 4α-phorbol 12,13-didecanoate increased cationic currents, intracellular Ca2+, and the CBF in the absence of a viscous load. Therefore, TRPV4 emerges as a candidate to participate in the coupling of fluid viscosity changes to the generation of the Ca2+ signal required for the autoregulation of CBF.
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影响因子：
4.8
作者：
Suzuki, M;Mizuno, A;Imai, M
通讯作者：
Imai, M
DOI：
10.1164/ajrccm/144.5.1091
发表时间：
1991-11-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
JOHNSON, NT;VILLALON, M;VERDUGO, P
通讯作者：
VERDUGO, P
DOI：
10.1046/j.1432-1327.2000.01615.x
发表时间：
2000-09-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Pedersen, S;Lambert, IH;Hoffmann, EK
通讯作者：
Hoffmann, EK
影响因子：
2.4
作者：
Rutllant, J;López-Béjar, M;López-Gatius, F
通讯作者：
López-Gatius, F
影响因子：
3.5
作者：
Wissenbach, U;Bödding, M;Flockerzi, V
通讯作者：
Flockerzi, V