Induction of the receptor for erythropoietin in murine erythroleukemia cells after dimethyl sulfoxide treatment.
Induction of the receptor for erythropoietin in murine erythroleukemia cells after dimethyl sulfoxide treatment.
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二甲亚砜处理后小鼠红白血病细胞中促红细胞生成素受体的诱导。
作者：
A. Tojo;Hiromi Fukamachi;Tsunehiro Saito;Masato Kasuga;Akio Urabe;F. Takaku
生物活性125 I标记的人重组促红细胞生成素（EPO）被用来证明在小鼠红白血病细胞克隆B8的细胞表面上的这种红细胞特异性造血生长因子的特异性受体。放射性碘标记的EPO与这些细胞的结合具有时间和温度依赖性、特异性、饱和性和可逆性。在二甲基亚砜诱导红系分化过程中，B8细胞在出现含血红蛋白的细胞之前，特异性125 I-EPO结合量迅速显着增加。饱和结合数据的Scatchard分析显示，B8细胞具有单一类别和低数量（350至650）的EPO受体/细胞，表观Kd为1.2至1.4 nM。此外，B8细胞上的EPO受体的数量增加了两倍，用DMSO诱导1天，但EPO对其受体的结合亲和力没有显着变化。用二琥珀酰亚胺辛二酸酯进行的亲和交联实验证明，在还原和非还原条件下，两种放射性标记组分的表观分子量分别为145，000和130，000。这两种成分的标记被抑制与未标记的EPO的细胞孵育。这些结果表明，一些小鼠红白血病细胞可能表达EPO受体作为红系分化标志物，其含有分子量为109，000和94，000的两种多肽。
Biologically active 125I-labeled human recombinant erythropoietin (EPO) was used to demonstrate specific receptors for this erythroid-specific hemopoietic growth factor on the cell surface of murine erythroleukemia cell clone B8. The binding of radioiodinated EPO to these cells was time and temperature dependent, specific, saturable, and reversible. During erythroid differentiation by dimethyl sulfoxide, B8 cells displayed a rapid and marked increase in the amount of specific 125I-EPO binding before the appearance of hemoglobin-containing cells. Scatchard analysis of the saturation binding data revealed that B8 cells had a single class and low number (350 to 650) of EPO receptors per cell with an apparent Kd of 1.2 to 1.4 nM. In addition, the number of EPO receptors on B8 cells was increased twice by induction with DMSO for 1 day, but the binding affinity of EPO toward its receptors did not change significantly. Affinity cross-linking experiments with disuccinimidyl suberate demonstrated two radiolabeled components with apparent molecular weights of 145,000 and 130,000 under both reducing and nonreducing conditions. Labeling of the two components was inhibited by incubation of cells with unlabeled EPO. These results suggest that some murine erythroleukemia cells potentially express EPO receptors as a differentiation marker of erythroid lineage, which contain two polypeptides with molecular weights of 109,000 and 94,000.
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分化诱导剂、粒细胞集落刺激因子与有反应但并非无反应的白血病细胞系结合。
DOI：
10.1073/pnas.81.12.3765
发表时间：
1984
影响因子：
11.1
作者：
Nicola,NA;Metcalf,D
通讯作者：
Metcalf,D
通过与弗兰德病毒感染的红细胞交联来鉴定促红细胞生成素受体。
DOI：
10.1073/pnas.84.11.3690
发表时间：
1987
影响因子：
11.1
作者：
Sawyer,ST;Krantz,SB;Luna,J
通讯作者：
Luna,J
DOI：
10.1073/pnas.82.22.7580
发表时间：
1985-01-01
影响因子：
11.1
作者：
LIN, FK;SUGGS, S;GOLDWASSER, E
通讯作者：
GOLDWASSER, E
弗兰德病毒感染的红细胞中促红细胞生成素的结合和受体介导的内吞作用。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Sawyer,ST;Krantz,SB;Goldwasser,E
通讯作者：
Goldwasser,E
促红细胞生成素与感染弗兰德病毒贫血株的脾细胞特异性结合。
DOI：
10.1073/pnas.81.23.7574
发表时间：
1984
影响因子：
11.1
作者：
Krantz,SB;Goldwasser,E
通讯作者：
Goldwasser,E