A protocol for full-rotation soft X-ray tomography of single cells.
A protocol for full-rotation soft X-ray tomography of single cells.
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DOI：
10.1016/j.xpro.2022.101176
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发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Weinhardt V
中科院分区：
文献类型：
--
作者：
Chen JH;Vanslembrouck B;Loconte V;Ekman A;Cortese M;Bartenschlager R;McDermott G;Larabell CA;Le Gros MA;Weinhardt V
该协议描述一步一步的样品制备，数据采集和细胞器的分割与软x射线断层扫描。它是专为在圆柱形样品支架上对标本进行全旋转数据采集的显微镜而设计的，例如先进光源的XM-2显微镜，LBNL；然而，它可以推广到类似的样品架设计为同步加速器和台式显微镜。有关使用和执行此配置文件的完整详细信息，请参阅。单细胞快速全旋转软x射线断层扫描生物细胞在玻璃毛细血管中低温保存x射线线性吸收系数定量校准主要细胞器分步分割方案描述了用软x射线断层扫描分步样品制备、数据采集和细胞器分割。它是专为在圆柱形样品支架上对标本进行全旋转数据采集的显微镜而设计的，例如先进光源的XM-2显微镜，LBNL；然而，它可以推广到类似的样品架设计为同步加速器和台式显微镜。
The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the Advanced Light Source, LBNL; however, it might be generalized for similar sample holder designs for both synchrotron and table-top microscopes. For complete details on the use and execution of this profile, please refer to. Rapid full-rotation soft X-ray tomography of single cells Cryopreservation of biological cells in glass capillaries Quantitative calibration of X-ray linear absorption coefficient Step-by-step segmentation of main cellular organelles The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the Advanced Light Source, LBNL; however, it might be generalized for similar sample holder designs for both synchrotron and table-top microscopes.
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影响因子：
3.4
作者：
Smith, Elizabeth A.;McDermott, Gerry;Larabell, Carolyn A.
通讯作者：
Larabell, Carolyn A.
影响因子：
3
作者：
Hanssen, Eric;Knoechel, Christian;Tilley, Leann
通讯作者：
Tilley, Leann
影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
影响因子：
3
作者：
Parkinson, Dilworth Y.;Knoechel, Christian;Yang, Chao;Larabell, Carolyn A.;Le Gros, Mark A.
通讯作者：
Le Gros, Mark A.
影响因子：
6.8
作者：
Larabell CA;Nugent KA
通讯作者：
Nugent KA