Imaging cellular architecture with X-rays.

Imaging cellular architecture with X-rays.
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DOI:
10.1016/j.sbi.2010.08.008
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发表时间:
2010-10
影响因子:
6.8
通讯作者:
Nugent KA
Nugent KA
中科院分区:
生物学2区
文献类型:
--
作者:
Larabell CA;Nugent KA

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生物样品的X射线成像正在迅速发展。在本文中,我们回顾了迄今为止在细胞结构的高分辨率成像的进展。特别是,我们调查软X射线断层扫描的进展,并认为该领域的时代即将到来,重要的生物学见解开始出现。然后,我们回顾了基于相干衍射的新想法。这些方法处于发展的早期阶段,但由于它们消除了对X射线光学器件的需要,因此能够提供比基于波带片的方法更好的空间分辨率。
X-ray imaging of biological samples is progressing rapidly. In this paper we review the progress to date in high resolution imaging of cellular architecture. In particular we survey the progress in soft X-ray tomography and argue that the field is coming of age and that important biological insights are starting to emerge. We then review the new ideas based on coherent diffraction. These methods are at a much earlier stage of development but, as they eliminate the need for X-ray optics, have the capacity to provide substantially better spatial resolution than zone plate based methods.
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