KIFC1-like motor protein associates with the cephalopod manchette and participates in sperm nuclear morphogenesis in Octopus tankahkeei.
KIFC1-like motor protein associates with the cephalopod manchette and participates in sperm nuclear morphogenesis in Octopus tankahkeei.
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KIFC1 样运动蛋白与头足类 Manchette 相关并参与章鱼精子核形态发生
DOI：
10.1371/journal.pone.0015616
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发表时间：
2010-12-20
期刊：
影响因子：
3.7
通讯作者：
Yang WX
中科院分区：
文献类型：
--
作者：
Wang W;Zhu JQ;Yu HM;Tan FQ;Yang WX
研究背景核形态发生是精子发生过程中最基本的细胞转化过程之一。在啮齿类动物中，基于微管的核周结构，manchette和C-末端驱动蛋白马达KIFC 1被认为在这一过程中起着至关重要的作用。章鱼的精子发生是一个很好的模型系统，以探索进化是否创造了一个头足类动物的原型哺乳动物manchette和KIFC 1依赖的精子核成形机械。方法/主要发现我们检测到的存在KIFC 1样蛋白在睾丸，肌肉和肝脏的O。Western Blot法测定。利用免疫荧光技术和免疫金电镜技术，追踪其在不同发育阶段精子细胞中的动态定位。KIFC 1样蛋白在精子发生的早期阶段不表达，当没有显著的形态学变化发生时，开始存在于早期精子细胞中，定位于中期和晚期精子细胞的核周围和核中，其中核被显著拉长和压缩，并集中在最终精子细胞的一端。此外，在核延伸和浓缩的马达蛋白的分布重叠的头足类对应的曼切特在一个显着的水平。结论/意义本研究结果支持该蛋白在O. tankahkeei可能是通过桥的manchette样的核周微管的细胞核，并协助特定货物的核质运输。这项研究代表了运动蛋白在头足类动物精子核成形中的作用的第一个描述。
Background Nuclear morphogenesis is one of the most fundamental cellular transformations taking place during spermatogenesis. In rodents, a microtubule-based perinuclear structure, the manchette, and a C-terminal kinesin motor KIFC1 are believed to play crucial roles in this process. Spermatogenesis in Octopus tankahkeei is a good model system to explore whether evolution has created a cephalopod prototype of mammalian manchette-based and KIFC1-dependent sperm nuclear shaping machinery. Methodology/Principal Findings We detected the presence of a KIFC1-like protein in the testis, muscle, and liver of O. tankahkeei by Western Blot. Then we tracked its dynamic localization in spermatic cells at various stages using Immunofluorescence and Immunogold Electron Microscopy. The KIFC1-like protein was not expressed at early stages of spermatogenesis when no significant morphological changes occur, began to be present in early spermatid, localized around and in the nucleus of intermediate and late spermatids where the nucleus was dramatically elongated and compressed, and concentrated at one end of final spermatid. Furthermore, distribution of the motor protein during nuclear elongation and condensation overlapped with that of the cephalopod counterpart of manchette at a significant level. Conclusions/Significance The results support the assumption that the protein is actively involved in sperm nuclear morphogenesis in O. tankahkeei possibly through bridging the manchette-like perinuclear microtubules to the nucleus and assisting in the nucleocytoplasmic trafficking of specific cargoes. This study represents the first description of the role of a motor protein in sperm nuclear shaping in cephalopod.
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