Spatially resolved correlative microscopy and microbial identification reveal dynamic depth- and mineral-dependent anabolic activity in salt marsh sediment.
Spatially resolved correlative microscopy and microbial identification reveal dynamic depth- and mineral-dependent anabolic activity in salt marsh sediment.
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DOI：
10.1111/1462-2920.15667
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发表时间：
2021-08
影响因子：
5.1
通讯作者：
Hatzenpichler R
中科院分区：
文献类型：
--
作者：
Marlow J;Spietz R;Kim KY;Ellisman M;Girguis P;Hatzenpichler R
滨海盐沼是生态地球化学循环的重要场所，也是研究复杂微生物群落结构的理想系统。在这里，我们澄清微生物和它们的矿物学环境之间的结构-功能关系，揭示以前未描述的代谢活动模式和精确的空间排列在盐沼沉积物。在与并入新生物质的非规范氨基酸原位孵育3.7天后，将样品树脂包埋并通过相关荧光和电子显微镜进行分析，以绘制合成代谢活性和非活性生物体与矿物质颗粒的微尺度排列。通过荧光激活细胞分选和16 S rRNA基因测序检查平行沉积物样品，以将合成代谢活性与分类身份联系起来。这两种方法都表明，随着盐沼沉积物深度的增加，合成代谢活性细胞的比例迅速下降，从顶部厘米处的约60%下降到2至10厘米处的9.4%-22.4%。从顶部到底部，最突出的活性群落成员从硫循环光养聚生体转移到可能氧化有机化合物的假定硫酸盐还原菌，再到发酵谱系。相关显微镜显示，在非石英矿物（包括金红石、正长石和斜长石）周围有更丰富的（合成代谢更活跃的）生物。微生物-矿物关系似乎是生物地球化学循环的动态和环境依赖性仲裁者。
Coastal salt marshes are key sites of biogeochemical cycling and ideal systems in which to investigate the community structure of complex microbial communities. Here, we clarify structural–functional relationships among microorganisms and their mineralogical environment, revealing previously undescribed metabolic activity patterns and precise spatial arrangements within salt marsh sediment. Following 3.7‐day in situ incubations with a non‐canonical amino acid that was incorporated into new biomass, samples were resin‐embedded and analysed by correlative fluorescence and electron microscopy to map the microscale arrangements of anabolically active and inactive organisms alongside mineral grains. Parallel sediment samples were examined by fluorescence‐activated cell sorting and 16S rRNA gene sequencing to link anabolic activity to taxonomic identity. Both approaches demonstrated a rapid decline in the proportion of anabolically active cells with depth into salt marsh sediment, from ~60% in the top centimetre to 9.4%–22.4% between 2 and 10 cm. From the top to the bottom, the most prominent active community members shifted from sulfur cycling phototrophic consortia, to putative sulfate‐reducing bacteria likely oxidizing organic compounds, to fermentative lineages. Correlative microscopy revealed more abundant (and more anabolically active) organisms around non‐quartz minerals including rutile, orthoclase and plagioclase. Microbe–mineral relationships appear to be dynamic and context‐dependent arbiters of biogeochemical cycling.
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影响因子：
1.4
作者：
Apprill, Amy;McNally, Sean;Weber, Laura
通讯作者：
Weber, Laura
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影响因子：
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