Novel Correlation between TGF-β1/-β3 and Hormone Receptors in the Human Corneal Stroma.
Novel Correlation between TGF-β1/-β3 and Hormone Receptors in the Human Corneal Stroma.
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人角膜基质中TGF-β1/-β3与激素受体之间的新型相关性。
DOI：
10.3390/ijms241713635
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发表时间：
2023-09-04
影响因子：
5.6
通讯作者：
Karamichos, Dimitrios
中科院分区：
文献类型：
--
作者：
Choi, Alexander J.;Hefley, Brenna S.;Nicholas, Sarah E.;Cunningham, Rebecca L.;Karamichos, Dimitrios
关键词：
This study investigated the interplay between transforming growth factor beta (TGF-β1/T1 and TGF-β3/T3), and sex hormone receptors using our 3D in vitro cornea stroma model. Primary human corneal fibroblasts (HCFs) from healthy donors were plated in transwells at 106 cells/well and cultured for four weeks. HCFs were supplemented with stable vitamin C (VitC) and stimulated with T1 or T3. 3D construct proteins were analyzed for the androgen receptor (AR), progesterone receptor (PR), estrogen receptor alpha (ERα) and beta (ERβ), luteinizing hormone receptor (LHR), follicle-stimulating hormone receptor (FSHR), gonadotropin-releasing hormone receptor (GnRHR), KiSS1-derived peptide receptor (KiSS1R/GPR54), and follicle-stimulating hormone subunit beta (FSH-B). In female constructs, T1 significantly upregulated AR, PR, ERα, FSHR, GnRHR, and KiSS1R. In male constructs, T1 significantly downregulated FSHR and FSH-B and significantly upregulated ERα, ERβ, and GnRHR. T3 caused significant upregulation in expressions PR, ERα, ERβ, LHR, FSHR, and GNRHR in female constructs, and significant downregulation of AR, ERα, and FSHR in male constructs. Semi-quantitative Western blot findings present the interplay between sex hormone receptors and TGF-β isoforms in the corneal stroma, which is influenced by sex as a biological variable (SABV). Additional studies are warranted to fully delineate their interactions and signaling mechanisms.
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影响因子：
3.3
作者：
Karamichos, D.;Hutcheon, A. E. K.;Zieske, J. D.
通讯作者：
Zieske, J. D.
DOI：
10.22088/ijmcm.bums.10.4.234
发表时间：
2021
影响因子：
--
作者：
通讯作者：
--
影响因子：
2.7
作者：
Shah TA;Rogers MB
通讯作者：
Rogers MB
循环睾丸激素是运动表现性别差异的激素基础。
影响因子：
20.3
作者：
Handelsman DJ;Hirschberg AL;Bermon S
通讯作者：
Bermon S
影响因子：
--
作者：
Bajwa SK;Singh S;Bajwa SJ
通讯作者：
Bajwa SJ