The effect of BMP-mimetic peptide tethering bioinks on the differentiation of dental pulp stem cells (DPSCs) in 3D bioprinted dental constructs.
The effect of BMP-mimetic peptide tethering bioinks on the differentiation of dental pulp stem cells (DPSCs) in 3D bioprinted dental constructs.
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DOI：
10.1088/1758-5090/ab9492
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发表时间：
2020-07-01
期刊：
影响因子：
9
通讯作者：
Lee SJ
中科院分区：
文献类型：
--
作者：
Park JH;Gillispie GJ;Copus JS;Zhang W;Atala A;Yoo JJ;Yelick PC;Lee SJ
这项研究的目标是使用3D生物打印技术创建包含人牙髓干细胞(HDPSCs)的生物工程牙科结构。为了实现这一目标，我们首先开发了一种新型的骨形态发生蛋白(BMP)多肽-栓系生物墨水配方，并检测了其流变性、可印刷性和生物打印构建体的结构稳定性。其次，我们通过测量细胞活力、增殖和基因表达，以及组织学和免疫荧光分析，评估了hDPSCs在生物打印牙科结构中的存活和分化。我们的结果表明，多肽成功地偶联到基于明胶甲基丙烯酸酯(GelMA)的生物墨水配方中。我们确定在体外细胞培养3周后，超过50%的多肽仍保留在生物印迹构建体中。人的DPSC活性在打印过程后立即在生物打印的构建物中为90%。茜素红染色显示，BMP多肽构建组的钙化程度最高，高于生长介质组、成骨介质组和非BMP多肽构建组。此外，免疫荧光和定量逆转录聚合酶链式反应(qRT-PCR)分析表明，牙本质涎磷蛋白(DSPP)和骨钙素(OCN)在BMP多肽牙科结构中有良好的表达。综上所述，这些结果有力地表明，BMP多肽结合的生物墨水可以加速hDPSCs在三维生物打印牙科结构中的分化。
The goal of this study was to use 3D bioprinting technology to create a bioengineered dental construct containing human dental pulp stem cells (hDPSCs). To accomplish this, we first developed a novel bone morphogenetic protein (BMP) peptide-tethering bioink formulation and examined its rheological properties, its printability, and the structural stability of the bioprinted construct. Second, we evaluated the survival and differentiation of hDPSCs in the bioprinted dental construct by measuring cell viability, proliferation, and gene expression, as well as histological and immunofluorescent analyses. Our results showed that the peptide conjugation into the gelatin methacrylate (GelMA)-based bioink formulation was successfully performed. We determined that greater than 50% of the peptides remained in the bioprinted construct after 3 weeks in vitro cell culture. Human DPSC viability was >90% in the bioprinted constructs immediately after the printing process. Alizarin Red staining showed that the BMP peptide construct group exhibited the highest calcification as compared to the growth medium, osteogenic medium, and non-BMP peptide construct groups. In addition, immunofluorescent and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analyses showed robust expression of dentin sialophosphoprotein (DSPP) and osteocalcin (OCN) in the BMP peptide dental constructs. Together, these results strongly suggested that BMP peptide-tethering bioink could accelerate the differentiation of hDPSCs in 3D bioprinted dental constructs.
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影响因子：
9
作者：
Gao T;Gillispie GJ;Copus JS;Pr AK;Seol YJ;Atala A;Yoo JJ;Lee SJ
通讯作者：
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通讯作者：
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作者：
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