Mapping sex differences in the effects of protein and carbohydrates on lifespan and reproduction in Drosophila melanogaster: is measuring nutrient intake essential?
Mapping sex differences in the effects of protein and carbohydrates on lifespan and reproduction in Drosophila melanogaster: is measuring nutrient intake essential?
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DOI：
10.1007/s10522-022-09953-2
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发表时间：
2022-03
期刊：
影响因子：
4.5
通讯作者：
Hunt J
中科院分区：
文献类型：
--
作者：
Carey MR;Archer CR;Rapkin J;Castledine M;Jensen K;House CM;Hosken DJ;Hunt J
了解饮食如何影响生殖和生存是进化生物学的中心目标。尽管这种关系可能在性别之间有所不同，但我们缺乏有关饮食与男性生殖特征之间关系的数据。这种普遍模式的一个例外是黑腹果蝇（Drosophila melanogaster），它的雄性饮食摄入量是用毛细管喂食器（CAFE）方法量化的。然而，CAFE喂养会降低黑腹d.p anogaster的存活和繁殖，因此可能会扭曲饮食适应性结果。在这里，我们使用营养的几何框架来创建营养景观，绘制出D. melanogaster的蛋白质、碳水化合物、寿命和繁殖之间的性别特异性关系。我们不是用消费数据创建景观，而是将特征映射到40种以琼脂为基础的饮食的营养成分上，从而产生广泛的营养空间覆盖。我们发现，低蛋白、高碳水化合物混合物（~ 1P:15.9C）的雄性和雌性寿命最长。这种营养比例也能最大化雄性的繁殖率，但雌性需要更多的蛋白质来最大化每日的繁殖力（p:1.22C）。这些结果与CAFE试验结果一致。然而，与CAFE相比，本文采用的方法提高了雌性的适合度，而琼脂饲喂与CAFE饲喂对雄性适合度性状的影响取决于实验饲料的营养成分。我们建议，在不测量个人营养摄入量的情况下，可以制作信息丰富的营养景观，并且在许多情况下，这可能比使用CAFE方法更可取。最合适的方法将取决于所研究的问题和物种，但这里采用的方法具有在饮食摄入量难以量化时创造营养景观的优势。在线版本包含补充材料，可在10.1007/s10522-022-09953-2获得。
Understanding how diet affects reproduction and survival is a central aim in evolutionary biology. Although this relationship is likely to differ between the sexes, we lack data relating diet to male reproductive traits. One exception to this general pattern is Drosophila melanogaster, where male dietary intake was quantified using the CApillary FEeder (CAFE) method. However, CAFE feeding reduces D. melanogaster survival and reproduction, so may distort diet-fitness outcomes. Here, we use the Geometric Framework of Nutrition to create nutrient landscapes that map sex-specific relationships between protein, carbohydrate, lifespan and reproduction in D. melanogaster. Rather than creating landscapes with consumption data, we map traits onto the nutrient composition of forty agar-based diets, generating broad coverage of nutrient space. We find that male and female lifespan was maximised on low protein, high carbohydrate blends (~ 1P:15.9C). This nutrient ratio also maximised male reproductive rates, but females required more protein to maximise daily fecundity (1P:1.22C). These results are consistent with CAFE assay outcomes. However, the approach employed here improved female fitness relative to CAFE assays, while effects of agar versus CAFE feeding on male fitness traits depended on the nutrient composition of experimental diets. We suggest that informative nutrient landscapes can be made without measuring individual nutrient intake and that in many cases, this may be preferable to using the CAFE approach. The most appropriate method will depend on the question and species being studied, but the approach adopted here has the advantage of creating nutritional landscapes when dietary intake is hard to quantify. The online version contains supplementary material available at 10.1007/s10522-022-09953-2.
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DOI：
10.1073/pnas.0702726104
发表时间：
2007-05-15
影响因子：
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作者：
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DOI：
10.1098/rspb.2014.2144
发表时间：
2015-03-07
影响因子：
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