Reorganization of Sleep by Temperature in Drosophila Requires Light, the Homeostat, and the Circadian Clock.
Reorganization of Sleep by Temperature in Drosophila Requires Light, the Homeostat, and the Circadian Clock.
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果蝇中温度按温度进行睡眠重组需要光，体内平移和昼夜节律。
DOI：
10.1016/j.cub.2016.02.011
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发表时间：
2016-04-04
期刊：
影响因子：
9.2
通讯作者：
Griffith, Leslie C.
中科院分区：
文献类型：
--
作者：
Parisky, Katherine M.;Rivera, Jose L. Agosto;Donelson, Nathan C.;Kotecha, Sejal;Griffith, Leslie C.
环境温度升高会重组果蝇的睡眠模式，就像人类对热的反应一样，增加白天的睡眠，减少夜间的睡眠。核心昼夜节律基因的突变阻止了白天睡眠的立即增加，但不能阻止夜间睡眠的热刺激减少，当动物处于光：暗周期时。per01果蝇增加白天睡眠的能力：黑暗可以通过在LNv或DN1p时钟细胞中表达PER来拯救，并且不需要拯救运动节律。长时间的高温暴露会使稳态器在夜间睡眠不足的情况下维持白天的睡眠。在恒定的黑暗中，所有基因型在主观白天的温度变化反应中显示出睡眠的立即减少，这意味着没有光输入揭示了温度升高的时钟独立的促醒效应。有趣的是，温度对夜间睡眠的影响在恒定的黑暗中减弱，而cryOUT突变体在光：暗中减弱，这表明它们依赖于前一天光线的存在。相比之下，所有基因型的果蝇在一天中的任何时候都保持在恒定的光照下，以应对高温，这表明光照的存在可以逆转正常的夜间反应。因此，温度对睡眠的影响反映了光、稳态器和生物钟组成部分的协调调节，使动物能够重新组织睡眠模式以应对高温，并大致保持睡眠总量。
Increasing ambient temperature reorganizes the Drosophila sleep pattern in a way similar to the human response to heat, increasing daytime sleep while decreasing nighttime sleep. Mutation of core circadian genes blocks the immediate increase in daytime sleep, but not the heat-stimulated decrease in nighttime sleep, when animals are in a light:dark cycle. The ability of per01 flies to increase daytime sleep in light:dark can be rescued by expression of PER in either LNv or DN1p clock cells and does not require rescue of locomotor rhythms. Prolonged heat exposure engages the homeostat to maintain daytime sleep in the face of nighttime sleep loss. In constant darkness, all genotypes show an immediate decrease in sleep in response to temperature shift during the subjective day, implying that the absence of light input uncovers a clock-independent proarousal effect of increased temperature. Interestingly, the effects of temperature on nighttime sleep are blunted in constant darkness and in cryOUT mutants in light:dark, suggesting that they are dependent on the presence of light the previous day. In contrast, flies of all genotypes kept in constant light sleep more at all times of day in response to high temperature, indicating that the presence of light can invert the normal nighttime response to increased temperature. The effect of temperature on sleep thus reflects coordinated regulation by light, the homeostat, and components of the clock, allowing animals to reorganize sleep patterns in response to high temperature with rough preservation of the total amount of sleep.
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影响因子：
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作者：
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DOI：
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发表时间：
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影响因子：
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