miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2.
miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2.
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DOI：
10.1177/15330338211041193
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发表时间：
2021-01
影响因子：
2.8
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Chen Z;Ying J;Shang W;Ding D;Guo M;Wang H
microRNA-342-3p在肿瘤发生发展中起着重要作用。然而，microRNA-342-3p在非小细胞肺癌中的表达模式和作用仍然知之甚少。在当前的研究中，我们通过功能获得和功能丧失分析探讨了microRNA-342-3p在非小细胞肺癌中的作用和潜在机制。我们采用定量逆转录聚合酶链反应和western blotting检测了microRNA-342-3p在非小细胞肺癌和b细胞淋巴瘤-2中的表达水平。此外，我们利用小干扰RNA和RNA模拟物分析了microRNA-342-3p在非小细胞肺癌细胞中的功能和潜在机制。采用荧光素酶报告基因实验，评估microRNA-342-3p靶向b细胞淋巴瘤-2的5 ' -非翻译区直接结合位点。我们发现microRNA-342-3p在非小细胞肺癌细胞和组织中的表达明显低于正常细胞和组织。在H1975、H460和H226细胞中，上调microRNA-342-3p抑制细胞增殖，促进细胞凋亡。microRNA-342-3p在非小细胞肺癌细胞中的过表达导致b细胞淋巴瘤-2细胞mRNA和蛋白水平下调。因此，b细胞淋巴瘤-2被确定为microRNA-342-3p的直接靶点。这些发现表明，microRNA-342-3p通过抑制b细胞淋巴瘤-2的表达来抑制非小细胞肺癌的生长，这表明microRNA-342-3p可能是治疗非小细胞肺癌的潜在靶点。
microRNA-342-3p plays an important role in tumor occurrence and development. However, the expression pattern and roles of microRNA-342-3p in nonsmall cell lung cancer remain poorly understood. In the current study, we explored the roles and underlying mechanisms of microRNA-342-3p in nonsmall cell lung cancer via gain- and loss-of-function analyses. We used quantitative reverse-transcription-polymerase chain reaction and western blotting assays to measure the expression levels of microRNA-342-3p in nonsmall-cell lung cancer and B-cell lymphoma-2. Furthermore, we used small interfering RNA and RNA mimics to analyze the functions and underlying mechanisms of microRNA-342-3p in nonsmall cell lung cancer cells. A luciferase reporter assay was performed to evaluate the direct binding site of the 5′-untranslated region of B-cell lymphoma-2 targeted by microRNA-342-3p. We found that the expression of microRNA-342-3p was significantly lower in nonsmall cell lung cancer cells and tissues than in normal cells and tissues. The upregulation of microRNA-342-3p suppressed cell proliferation while promoting apoptosis in H1975, H460, and H226 cells. The overexpression of microRNA-342-3p in nonsmall cell lung cancer cells led to the downregulation of mRNA and protein levels in B-cell lymphoma-2 cells. Thus, B-cell lymphoma-2 was identified as a direct target of microRNA-342-3p. These findings indicate that microRNA-342-3p inhibits the growth of nonsmall cell lung cancer by repressing the expression of B-cell lymphoma-2, which suggests that microRNA-342-3p could be a potential target for the treatment of nonsmall cell lung cancer.
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