Ebp2 and Brx1 function cooperatively in 60S ribosomal subunit assembly in Saccharomyces cerevisiae.
Ebp2 and Brx1 function cooperatively in 60S ribosomal subunit assembly in Saccharomyces cerevisiae.
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DOI：
10.1093/nar/gks057
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发表时间：
2012-05
影响因子：
14.9
通讯作者：
Mizuta K
中科院分区：
文献类型：
--
作者：
Shimoji K;Jakovljevic J;Tsuchihashi K;Umeki Y;Wan K;Kawasaki S;Talkish J;Woolford JL Jr;Mizuta K
酵母蛋白Ebp2在60S核糖体亚基产生的早期阶段是必需的。为了寻找Ebp2起作用的辅助因子或其作用的底物，我们筛选了具有Ebp2 -14突变的合成致死（sl）突变体。发现了4种不同的60S核糖体亚基组装因子Brx1突变等位基因。为了研究双突变体的缺陷，我们构建了符合ebp2-14 brx1-合成致死表型的菌株。这些ebp2-14 brx1突变体在27S pre-rRNA的加工和60S亚基的产生上存在缺陷，而每个突变体都没有缺陷。Ebp2和Brx1表现出强烈的双杂交相互作用，这种相互作用被Brx1和Ebp2突变的某些组合所消除。在一个这样的突变体中，Ebp2和Brx1仍然可以与前核糖体结合，但亚基成熟受到干扰。Ebp2或Brx1的缺失表明，Brx1需要Ebp2才能与前核糖体稳定结合，而Ebp2并不依赖于Brx1的存在进入前核糖体。这些结果表明60S核糖体亚基的组装需要Ebp2与Brx1的合作，以及存在于核糖体前的其他分子，可能包括与Brx1和Ebp2组装亚复合物中发现的一些分子。
The yeast protein Ebp2 is required for early steps in production of 60S ribosomal subunits. To search for cofactors with which Ebp2 functions, or substrates on which it acts, we screened for mutants that were synthetically lethal (sl) with the ebp2-14 mutation. Four different mutant alleles of the 60S ribosomal subunit assembly factor Brx1 were found. To investigate defects of the double mutant, we constructed strains conditional for the ebp2-14 brx1- synthetic lethal phenotype. These ebp2-14 brx1 mutants were defective in processing of 27S pre-rRNA and production of 60S subunits, under conditions where each single mutant was not. Ebp2 and Brx1 exhibit a strong two-hybrid interaction, which is eliminated by some combinations of brx1 and ebp2 mutations. In one such mutant, Ebp2 and Brx1 can still associate with pre-ribosomes, but subunit maturation is perturbed. Depletion of either Ebp2 or Brx1 revealed that Brx1 requires Ebp2 for its stable association with pre-ribosomes, but Ebp2 does not depend on the presence of Brx1 to enter pre-ribosomes. These results suggest that assembly of 60S ribosomal subunits requires cooperation of Ebp2 with Brx1, together with other molecules present in pre-ribosomes, potentially including several found in assembly subcomplexes with Brx1 and Ebp2.
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影响因子：
5.3
作者：
Miles, TD;Jakovljevic, J;Woolford, JL
通讯作者：
Woolford, JL
影响因子：
11.4
作者：
HENRY, Y;WOOD, H;TOLLERVEY, D
通讯作者：
TOLLERVEY, D
DOI：
10.1073/pnas.91.2.659
发表时间：
1994-01-18
影响因子：
11.1
作者：
CHU, S;ARCHER, RH;LINDAHL, L
通讯作者：
LINDAHL, L
影响因子：
64.5
作者：
KOSHLAND, D;KENT, JC;HARTWELL, LH
通讯作者：
HARTWELL, LH
影响因子：
3.5
作者：
Miyoshi, K;Shirai, C;Mizuta, K
通讯作者：
Mizuta, K