WOX5 suppresses CYCLIN D activity to establish quiescence at the center of the root stem cell niche.
WOX5 suppresses CYCLIN D activity to establish quiescence at the center of the root stem cell niche.
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DOI：
10.1016/j.cub.2014.07.019
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发表时间：
2014-08-18
期刊：
影响因子：
9.2
通讯作者：
Murray, James A. H.
中科院分区：
文献类型：
--
作者：
Forzani, Celine;Aichinger, Ernst;Sornay, Emily;Willemsen, Viola;Laux, Thomas;Dewitte, Walter;Murray, James A. H.
在拟南芥中，干细胞为根系生长提供新细胞。它们围绕着一组缓慢分裂的细胞，称为静止中心（QC），它们一起形成干细胞生态位（SCN）。QC作为SCN的信号传导中心，抑制周围干细胞的分化，并提供能够替代受损干细胞的细胞池。干细胞的维持依赖于转录因子wuschelrelated HOMEOBOX 5 (WOX5)，该转录因子在QC中特异性表达。然而，WOX5促进干细胞命运的分子机制以及WOX5是否调控QC的增殖尚不清楚。在这里，我们揭示了WOX5在抑制QC细胞分裂从而建立静止的新作用。WOX5与CYCD3；3/CYCD1；两者都促进新生小柱的细胞增殖。wox5突变体中发生的额外QC分裂在与D型细胞周期蛋白CYCD3的突变体组合中被抑制；3和CYCD1；此外，CYCD3异位表达；3在QC中足以诱导QC中的细胞分裂。因此WOX5抑制QC分裂，否则CYCD3会促进QC分裂；3和CYCD1；1，部分通过与CYCD3相互作用；3启动子抑制CYCD3；3 . QC中的表达。因此，我们提出WOX5通过排除QC中的CYCD活性来启动和维持QC的静止。WOX5抑制根干细胞小生境中心分裂启动静止WOX5抑制静止中心CYCD表达限制细胞分裂WOX5与CYCD3结合；3启动子CYCD3；3和CYCD1；Forzani等揭示了WOX5转录因子在拟南芥根干细胞生态位中心启动和维持静止的关键作用。他们证明WOX5通过阻止D型细胞周期蛋白CYCD3的表达来局部抑制细胞分裂；3个在静止的中心。
In Arabidopsis, stem cells maintain the provision of new cells for root growth. They surround a group of slowly dividing cells named the quiescent center (QC), and, together, they form the stem cell niche (SCN). The QC acts as the signaling center of the SCN, repressing differentiation of the surrounding stem cells and providing a pool of cells able to replace damaged stem cells. Maintenance of the stem cells depends on the transcription factor WUSCHEL-RELATED HOMEOBOX 5 (WOX5), which is specifically expressed in the QC. However, the molecular mechanisms by which WOX5 promotes stem cell fate and whether WOX5 regulates proliferation of the QC are unknown. Here, we reveal a new role for WOX5 in restraining cell division in the cells of the QC, thereby establishing quiescence. In contrast, WOX5 and CYCD3;3/CYCD1;1 both promote cell proliferation in the nascent columella. The additional QC divisions occurring in wox5 mutants are suppressed in mutant combinations with the D type cyclins CYCD3;3 and CYCD1;1. Moreover, ectopic expression of CYCD3;3 in the QC is sufficient to induce cell division in the QC. WOX5 thus suppresses QC divisions that are otherwise promoted by CYCD3;3 and CYCD1;1, in part by interacting with the CYCD3;3 promoter to repress CYCD3;3 expression in the QC. Therefore, we propose a specific role for WOX5 in initiating and maintaining quiescence of the QC by excluding CYCD activity from the QC. WOX5 prevents divisions at the root stem cell niche center to initiate quiescence WOX5 suppresses CYCD expression in the quiescent center to restrict cell divisions WOX5 binds to the CYCD3;3 promoter CYCD3;3 and CYCD1;1 stimulate division during formation of the columella Forzani et al. reveal a critical role for the WOX5 transcription factor in initiating and maintaining quiescence at the center of the root stem cell niche in Arabidopsis. They demonstrate that WOX5 locally suppresses cell division by preventing expression of the D type cyclin CYCD3;3 in the quiescent center.
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