Parabiosis reveals leukocyte dynamics in the kidney.
Parabiosis reveals leukocyte dynamics in the kidney.
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DOI：
10.1038/labinvest.2017.130
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
George JF
中科院分区：
文献类型：
--
作者：
Lever JM;Yang Z;Boddu R;Adedoyin OO;Guo L;Joseph R;Traylor AM;Agarwal A;George JF
肾脏的免疫细胞区室参与器官发育和体内平衡以及许多病理状况。在肾小管和间质细胞死亡的情况下，驱动肾内免疫反应的机制知之甚少。然而，已知组织驻留的白细胞与循环细胞相比具有发挥不同作用的潜力。我们在C57 BL/6 CD 45同源和GFP转基因小鼠中使用联体共生模型，以更好地了解肾脏中免疫细胞的动态。我们发现，F4/80 Hi肾内巨噬细胞表现出最小的交换与外周循环在两种模型的联体，无论小鼠连接4或16周。其他肾内炎性细胞表现出与健康肾脏中的循环免疫细胞池几乎完全交换，表明先天性和适应性免疫细胞在正常生理过程中广泛地通过肾间质运输。中性粒细胞、树突状细胞、F4/80低巨噬细胞、T细胞、B细胞和NK细胞从循环免疫细胞库中更新。然而，一部分双阴性T细胞（CD 4 − CD 8 −）和NKT细胞是长寿的或组织驻留的。本研究提供了白细胞亚群驻留在肾组织中的直接证据，与外周血的交换极少或无。此外，数据表明其他亚群通过未发炎组织持续交换。
The immune cellular compartment of the kidney is involved in organ development and homeostasis as well as in many pathological conditions. Little is known about the mechanisms that drive intrarenal immune responses in the presence of renal tubular and interstitial cell death. However, it is known that tissue resident leukocytes have the potential to play distinct roles compared with circulating cells. We used a parabiosis model in C57BL/6 CD45 congenic and GFP transgenic mice to better understand the dynamics of immune cells in the kidney. We found F4/80Hi intrarenal macrophages exhibit minimal exchange with the peripheral circulation in two models of parabiosis, whether mice were attached for 4 or 16 weeks. Other intrarenal inflammatory cells demonstrate near total exchange with the circulating immune cell pool in healthy kidneys, indicating that innate and adaptive immune cells extensively traffic through the kidney interstitium during normal physiology. Neutrophils, dendritic cells, F4/80Low macrophages, T cells, B cells, and NK cells are renewed from the circulating immune cell pool. However, a fraction of double negative T (CD4− CD8−) and NKT cells are long-lived or tissue resident. This study provides direct evidence of leukocyte subpopulations that are resident in the renal tissue, with minimal to no exchange with the peripheral blood. In addition, the data demonstrate continual exchange of other subpopulations through uninflamed tissue.
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对抗生素小鼠的跨循环和细胞分布动力学。
影响因子：
5.6
作者：
Gibney, Barry C.;Chamoto, Kenji;Lee, Grace S.;Simpson, Dinee C.;Miele, Lino F.;Tsuda, Akira;Konerding, Moritz A.;Wagers, Amy;Mentzer, Steven J.
通讯作者：
Mentzer, Steven J.
DOI：
10.4049/jimmunol.1600281
发表时间：
2017-04-01
期刊：
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影响因子：
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作者：
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影响因子：
41.5
作者：
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通讯作者：
Bonventre, Joseph V.
影响因子：
3.2
作者：
Kopp JB
通讯作者：
Kopp JB
影响因子：
13.6
作者：
Ernandez T;Mayadas TN
通讯作者：
Mayadas TN