Cross-circulation and cell distribution kinetics in parabiotic mice.
Cross-circulation and cell distribution kinetics in parabiotic mice.
复制标题
对抗生素小鼠的跨循环和细胞分布动力学。
DOI：
10.1002/jcp.22796
复制
发表时间：
2012-02
影响因子：
5.6
通讯作者：
Mentzer, Steven J.
中科院分区：
文献类型：
--
作者：
Gibney, Barry C.;Chamoto, Kenji;Lee, Grace S.;Simpson, Dinee C.;Miele, Lino F.;Tsuda, Akira;Konerding, Moritz A.;Wagers, Amy;Mentzer, Steven J.
血源性有核细胞不仅参与炎症，而且参与组织修复和再生。由于祖细胞和干细胞群体在血液中的浓度低，这些细胞的循环动力学和组织分布在很大程度上是未知的。追踪细胞谱系的一个重要方法是使用荧光示踪剂和交叉循环的联体模型。在这里，我们研究了C57/B6 GFP+/野生型寄生虫的交叉循环和细胞分布动力学。联体后外周血的流式细胞术分析表明，没有证据表明基于细胞大小或表面特征的“联体屏障”;本研究中的所有外周血细胞亚群在14天内达到平衡。全血荧光分析表明，平均交换流速为16μl/hr或每小时循环血量的0.66%。外周淋巴器官的研究表明差异细胞分布动力学。一些亚群，如CD 8+和CD 11 c+，在淋巴结和脾脏中达到平衡，表明停留时间小于28天;相反，其他淋巴细胞亚群，如B220+和CD 4+细胞，在28天时尚未达到平衡。我们的结论是，联体共生可以提供重要的见解定义组织分布，停留时间，和再循环池使用荧光标记的细胞谱系。
Blood-borne nucleated cells participate not only in inflammation, but in tissue repair and regeneration. Because progenitor and stem cell populations have a low concentration in the blood, the circulation kinetics and tissue distribution of these cells is largely unknown. An important approach to tracking cell lineage is the use of fluorescent tracers and parabiotic models of cross-circulation. Here, we investigated the cross-circulation and cell distribution kinetics of C57/B6 GFP+/wild-type parabionts. Flow cytometry analysis of the peripheral blood after parabiosis demonstrated no evidence for a “parabiotic barrier” based on cell size or surface characterstics; all peripheral blood cell subpopulations in this study reached equilibrium within 14 days. Whole blood fluorescence analysis indicated that the mean exchange flow rate was 16μl/hr or 0.66% of the circulating blood volume per hour. Studies of peripheral lymphoid organs indicated differential cell distribution kinetics. Some subpopulations, such as CD8+ and CD11c+, equilibrated in both lymph nodes and spleen indicating a residence time less than 28 days; in contrast, other lymphocyte subpopulations, such as B220+ and CD4+ cells, had not yet reached equilibrium at 28 days. We conclude that parabiosis can provide important insights into defining tissue distribution, residence times, and recirculating pools using fluorochrome markers of cell lineage.
登录
查看更多内容
影响因子：
20.1
作者：
Aicher, Alexandra;Rentsch, Markus;Heeschen, Christopher
通讯作者：
Heeschen, Christopher
影响因子：
2.2
作者：
Su, M;He, CF;Mentzer, SJ
通讯作者：
Mentzer, SJ
影响因子：
--
作者：
HUFF, RL;TRAUTMAN, R;VANDYKE, DC
通讯作者：
VANDYKE, DC
影响因子：
4.4
作者：
West, CA;He, C;Mentzer, SJ
通讯作者：
Mentzer, SJ
影响因子：
--
作者：
Bunster, E;Meyer, RK
通讯作者：
Meyer, RK