Nsp9 and Nsp10 contribute to the fatal virulence of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China.
Nsp9 and Nsp10 contribute to the fatal virulence of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China.
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Nsp9和Nsp10导致中国出现的高致病性猪繁殖与呼吸综合征病毒具有致命毒力
DOI：
10.1371/journal.ppat.1004216
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发表时间：
2014-07
期刊：
影响因子：
6.7
通讯作者：
Yang H
中科院分区：
文献类型：
--
作者：
Li Y;Zhou L;Zhang J;Ge X;Zhou R;Zheng H;Geng G;Guo X;Yang H
由中国高致病性猪繁殖与呼吸综合征病毒（HP-PRRSV）引起的非典型猪繁殖与呼吸综合征（PRRS）自2006年爆发以来，给养猪业造成了巨大的经济损失。然而，迄今为止，病毒基因组内与HP-PRRSV致命毒力相关的区域仍然未知。在本研究中，我们产生了一系列全长感染性cDNA克隆，其在高致病性RvJXwn和低致病性RvHB-1/3.9之间具有交换的编码区。然后，系统分析了拯救的嵌合病毒的体外和体内复制以及对仔猪的致病性，并与其主链病毒进行了比较。首先，我们将两株病毒的5′UTR+ ORF 1a、ORF 1b和结构蛋白（SPs）编码区进行了互换，证明了非结构蛋白编码区（ORF 1b）与HP-PRRSV的致死性和体内外复制效率的提高直接相关。此外，我们在两种病毒之间分别替换了非结构蛋白（Nsp）9-、Nsp 10-、Nsp 11-和Nsp 12-编码区;或一起替换了Nsp 9-和Nsp 10-编码区;或同时替换了Nsp 9-、Nsp 10-和Nsp 11-编码区。本研究结果表明，HP-PRRSV Nsp 9和Nsp 10编码区共同作用与其体内外复制效率密切相关，并与其对仔猪的致病性和致死性增强有关。我们的研究结果表明，Nsp 9和Nsp 10共同贡献了中国出现的HP-PRRSV的致命毒力，有助于阐明这种病毒的致病机制。
Atypical porcine reproductive and respiratory syndrome (PRRS), which is caused by the Chinese highly pathogenic PRRS virus (HP-PRRSV), has resulted in large economic loss to the swine industry since its outbreak in 2006. However, to date, the region(s) within the viral genome that are related to the fatal virulence of HP-PRRSV remain unknown. In the present study, we generated a series of full-length infectious cDNA clones with swapped coding regions between the highly pathogenic RvJXwn and low pathogenic RvHB-1/3.9. Next, the in vitro and in vivo replication and pathogenicity for piglets of the rescued chimeric viruses were systematically analyzed and compared with their backbone viruses. First, we swapped the regions including the 5′UTR+ORF1a, ORF1b, and structural proteins (SPs)-coding region between the two viruses and demonstrated that the nonstructural protein-coding region, ORF1b, is directly related to the fatal virulence and increased replication efficiency of HP-PRRSV both in vitro and in vivo. Furthermore, we substituted the nonstructural protein (Nsp) 9-, Nsp10-, Nsp11- and Nsp12-coding regions separately; or Nsp9- and Nsp10-coding regions together; or Nsp9-, Nsp10- and Nsp11-coding regions simultaneously between the two viruses. Our results indicated that the HP-PRRSV Nsp9- and Nsp10-coding regions together are closely related to the replication efficiency in vitro and in vivo and are related to the increased pathogenicity and fatal virulence for piglets. Our findings suggest that Nsp9 and Nsp10 together contribute to the fatal virulence of HP-PRRSV emerging in China, helping to elucidate the pathogenesis of this virus.
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影响因子：
3.7
作者：
Guo B;Lager KM;Henningson JN;Miller LC;Schlink SN;Kappes MA;Kehrli ME Jr;Brockmeier SL;Nicholson TL;Yang HC;Faaberg KS
通讯作者：
Faaberg KS
影响因子：
5
作者：
Fang Y;Snijder EJ
通讯作者：
Snijder EJ
影响因子：
2.7
作者：
Allende R;Kutish GF;Laegreid W;Lu Z;Lewis TL;Rock DL;Friesen J;Galeota JA;Doster AR;Osorio FA
通讯作者：
Osorio FA
五对PRRSV强毒亲本/弱毒疫苗株的比较基因组分析
影响因子：
3.3
作者：
An, Tong-Qing;Tian, Zhi-Jun;Tong, Guang-Zhi
通讯作者：
Tong, Guang-Zhi
影响因子：
2.7
作者：
Gao, ZQ;Guo, X;Yang, HC
通讯作者：
Yang, HC