BnC15 and BnATA20, the different putative components, control anther development in Brassica napus L.
BnC15 and BnATA20, the different putative components, control anther development in Brassica napus L.
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BnC15 和 BnATA20 是不同的假定组分，控制甘蓝型油菜花药的发育。
DOI：
10.1016/j.gene.2012.07.028
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发表时间：
2012-10
期刊：
影响因子：
3.5
通讯作者：
Yang, Guangsheng
中科院分区：
文献类型：
--
作者：
Wan, Lili;Hu, Qin;Hong, Dengfeng;Yang, Guangsheng
在甘蓝型油菜中，雄性育性取决于花药中适当的细胞分化。然而，对调控花药细胞分化和功能的基因知之甚少。在此，我们报道了两个花器官特异性基因，BnC 15和BnATA 20，来自一个B。油菜双系Rs 1046 A/B花差减文库。原位杂交和实时荧光定量PCR分析表明，BnC 15和BnATA 20基因在花药中的表达模式不同，但在B中，这两个基因的沉默都是由于BnC 15和BnATA 20基因在花药中的表达模式不同而引起的。napus的反义抑制导致小孢子释放后花粉败育。光学和电子显微镜观察发现，缺乏质体，脂体和孢粉素分泌绒毡层导致外壁雕刻和形成的花粉衣的畸变。此外，小孢子最终被挤压成不规则的形状。通过对转基因植株的基因表达分析以及对bnc 15和bnata 20突变体与Rs 1046 A花药发育的比较表明，BnC 15和BnATA 20在控制Rs 1046 B育性的Rf基因下游受到正向调控，调控途径相同。这些结果支持了BnC 15和BnATA 20是控制绒毡层发育和外壁雕刻的遗传网络的重要组成部分的假设。
In Brassica napus, male fertility depends on proper cell differentiation in the anther. However, relatively little is known about the genes regulating anther cell differentiation and function. Here, we report two floral organ specific genes, BnC15 and BnATA20, derived from a B. napus two-line Rs1046A/B floral subtractive library. Although BnC15 and BnATA20 genes have a different expression pattern in anthers demonstrated by in situ hybridization and real-time PCR analysis, silencing of both genes in B. napus by antisense suppression resulted in pollen abortion after microspore release. Light and electron microscopy observation revealed the lack of plastoglobuli, lipid bodies and sporopollenin secreted from the tapetum leading to aberrations in exine sculpturing and the formation of a pollen coat. In addition, the microspores were squeezed to the irregular shape in the locule in the end. As shown by gene expression analysis in transgenic plants and the comparison of anther development between bnc15 or bnata20 mutants and Rs1046A, BnC15 and BnATA20 were positively regulated downstream of Rf gene controlling the fertility of Rs1046B in the same pathway. The results support the hypothesis that BnC15 and BnATA20 are crucial components of a genetic network that controls tapetum development and exine sculpturing.
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