Shifts in indigenous microbial communities during the anaerobic degradation of pentachlorophenol in upland and paddy soils from southern China
Shifts in indigenous microbial communities during the anaerobic degradation of pentachlorophenol in upland and paddy soils from southern China
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中国南方旱地和水稻土中五氯苯酚厌氧降解过程中本土微生物群落的变化
DOI：
10.1007/s11356-016-7562-8
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发表时间：
2016-09
影响因子：
5.8
通讯作者：
Yongkui Wang
中科院分区：
文献类型：
--
作者：
Yating Chen;Liang Tao;Ke Wu;Yongkui Wang
五氯苯酚（PCP）是一种常见的土壤持久性农药，已在世界范围内产生了严重的环境问题。因此，土壤土著微生物群落对五氯苯酚的厌氧降解越来越受到人们的关注。然而，关于功能性微生物以及与五氯苯酚降解相关的微生物群落的潜在变化的信息很少。在这项研究中，我们进行了一系列的实验，以确定哪些组成部分的土著微生物群落能够降解五氯苯酚的两种土地利用类型（高地和水稻土）在中国南方。结果表明，五氯酚在水稻土中的降解速率显著高于高地土壤。16S rRNA高通量测序结果表明，PCP污染土壤与对照土壤的微生物类群组成存在显著差异，其中不动杆菌属（Acinetobacter）、梭菌属（Clostridium）、粪球菌属（Coprococcus）、牛肝菌属（Oxidophilus）和沉降菌属（Sedimentilus）为PCP污染土壤中的优势菌群，不动杆菌属在PCP污染土壤中的富集程度最高（达52.2%），表明该菌属可能在PCP的降解过程中发挥重要作用。此外，还原三价铁的梭菌也可能参与五氯苯酚的降解。我们的数据进一步揭示了迄今为止未知的微生物包括粪球菌、牛肝菌和瘤胃梭菌对五氯苯酚的潜在降解代谢。总体而言，这些研究结果表明，土地利用类型可能会影响五氯苯酚厌氧降解率通过土著细菌种群的活动，并扩大我们的知识的细菌种群负责五氯苯酚的降解。
Pentachlorophenol (PCP) is a common persistent pesticide in soil that has generated a significant environmental problem worldwide. Therefore, anaerobic degradation of PCP by the soil indigenous microbial community has gained increasing attention. However, little information is available concerning the functional microorganisms and the potential shifts in the microbial community associated with PCP degradation. In this study, we conducted a set of experiments to determine which components of the indigenous microbial community were capable of degrading PCP in soils of two land use types (upland and paddy soils) in southern China. Our results showed that the PCP degradation rate was significantly higher in paddy soils than that in upland soils. 16S ribosomal RNA (rRNA) high-throughput sequencing revealed significant differences in microbial taxonomic composition between the soil with PCP and blank (soil without PCP) withAcinetobacter,Clostridium,Coprococcus,Oxobacter, andSedimentibacterdominating the PCP-affected communities.Acinetobacterwas also apparently enriched in the paddy soils with PCP (up to 52.2 %) indicated this genus is likely to play an important role in PCP degradation. Additionally, the Fe(III)-reducing bacteriaClostridiummay also be involved in PCP degradation. Our data further revealed hitherto unknown metabolisms of potential PCP degradation by microorganisms includingCoprococcus,Oxobacter, andRuminiclostridium. Overall, these findings indicated that land use types may affect the PCP anaerobic degradation rate via the activities of indigenous bacterial populations and extend our knowledge of the bacterial populations responsible for PCP degradation.
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影响因子：
4.4
作者：
T. Patel;K. G. Jure;G. Jones
通讯作者：
T. Patel;K. G. Jure;G. Jones
影响因子：
3.7
作者：
Nacke H;Thürmer A;Wollherr A;Will C;Hodac L;Herold N;Schöning I;Schrumpf M;Daniel R
通讯作者：
Daniel R
DOI：
10.1073/pnas.0808254105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Hartman, Wyatt H.;Richardson, Curtis J.;Bruland, Gregory L.
通讯作者：
Bruland, Gregory L.
影响因子：
3.6
作者：
Ashwani Sharma;I. Thakur;P. Dureja
通讯作者：
Ashwani Sharma;I. Thakur;P. Dureja
影响因子：
9.7
作者：
Xiaomin Li;Zhong Lin;Chunling Luo;Jing Bai;Yingtao Sun;Yongtao Li
通讯作者：
Xiaomin Li;Zhong Lin;Chunling Luo;Jing Bai;Yingtao Sun;Yongtao Li