Embryoid Body-Explant Outgrowth Cultivation from Induced Pluripotent Stem Cells in an Automated Closed Platform.
Embryoid Body-Explant Outgrowth Cultivation from Induced Pluripotent Stem Cells in an Automated Closed Platform.
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DOI：
10.1155/2016/7098987
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发表时间：
2016
影响因子：
--
通讯作者：
Umezawa A
中科院分区：
文献类型：
--
作者：
Tone H;Yoshioka S;Akiyama H;Nishimura A;Ichimura M;Nakatani M;Kiyono T;Toyoda M;Watanabe M;Umezawa A
细胞培养的自动化将促进具有一致质量的稳定细胞扩增。在本研究中，一个自动化的封闭式细胞培养系统的可行性“P 4C S”的胚状体（EB-）外植体生长培养作为外植体培养的模式案例进行了研究。在将诱导多能干细胞（iPSC）衍生的EB置于系统中之后，EB成功地粘附到培养物表面，并且在粘附的EB周围清楚地观察到细胞生长。在确认生长后，我们进行了传代培养操作，其中通过摇动培养瓶将分离的细胞简单地分散，导致均匀的细胞分布。这使得能够连续稳定地进行细胞扩增，导致细胞产量为3.1 × 107。在整个细胞培养实验中没有细菌污染的证据。因此，我们开发了EB-外植体生长细胞的自动化培养平台。
Automation of cell culture would facilitate stable cell expansion with consistent quality. In the present study, feasibility of an automated closed-cell culture system “P 4C S” for an embryoid body- (EB-) explant outgrowth culture was investigated as a model case for explant culture. After placing the induced pluripotent stem cell- (iPSC-) derived EBs into the system, the EBs successfully adhered to the culture surface and the cell outgrowth was clearly observed surrounding the adherent EBs. After confirming the outgrowth, we carried out subculture manipulation, in which the detached cells were simply dispersed by shaking the culture flask, leading to uniform cell distribution. This enabled continuous stable cell expansion, resulting in a cell yield of 3.1 × 107. There was no evidence of bacterial contamination throughout the cell culture experiments. We herewith developed the automated cultivation platform for EB-explant outgrowth cells.
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影响因子：
3
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
14
作者：
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通讯作者：
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影响因子：
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作者：
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