Human induced pluripotent stem cells on autologous feeders.
Human induced pluripotent stem cells on autologous feeders.
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DOI：
10.1371/journal.pone.0008067
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发表时间：
2009-12-02
期刊：
影响因子：
3.7
通讯作者：
Yamanaka S
中科院分区：
文献类型：
--
作者：
Takahashi K;Narita M;Yokura M;Ichisaka T;Yamanaka S
对于诱导多能干细胞(IPS)的治疗应用，实现无异种培养是至关重要的。以往的报道表明，人类胚胎干细胞可以在无饲养层的条件下维持。然而，缺乏饲养层细胞可能会对多能细胞造成不利的环境，并经常导致核型异常。人成纤维细胞可以替代动物饲养物作为人ES细胞的饲养细胞。然而，人们仍然必须关注这些非自体喂食者中是否存在不明病原体，如病毒和普恩。本报告证明了人诱导多能干细胞(IPS)可以在同基因的亲代饲养层细胞上建立和维持。我们测试了四个独立的人皮肤成纤维细胞，以保持iPS细胞的自我更新能力。所有被测试的成纤维细胞以及它们的条件培养液都能够维持iPS细胞的未分化状态和正常核型。此外，人iPS细胞可以在同基因的亲代成纤维细胞作为饲养者产生。这些iPS细胞以未分化的形态进行了19代以上的增殖。它们表达未分化的多能细胞标志，并可通过类胚体和畸胎瘤形成分化为所有三个胚层。这些结果表明，自体成纤维细胞不仅可以作为iPS细胞的来源，也可以作为饲养层。我们的结果为使用同基因成纤维细胞作为iPS细胞的饲养层来解决这一困境提供了可能性。这是建立临床级别iPS细胞的重要一步。
For therapeutic usage of induced Pluripotent Stem (iPS) cells, to accomplish xeno-free culture is critical. Previous reports have shown that human embryonic stem (ES) cells can be maintained in feeder-free condition. However, absence of feeder cells can be a hostile environment for pluripotent cells and often results in karyotype abnormalities. Instead of animal feeders, human fibroblasts can be used as feeder cells of human ES cells. However, one still has to be concerned about the existence of unidentified pathogens, such as viruses and prions in these non-autologous feeders. This report demonstrates that human induced Pluripotent Stem (iPS) cells can be established and maintained on isogenic parental feeder cells. We tested four independent human skin fibroblasts for the potential to maintain self-renewal of iPS cells. All the fibroblasts tested, as well as their conditioned medium, were capable of maintaining the undifferentiated state and normal karyotypes of iPS cells. Furthermore, human iPS cells can be generated on isogenic parental fibroblasts as feeders. These iPS cells carried on proliferation over 19 passages with undifferentiated morphologies. They expressed undifferentiated pluripotent cell markers, and could differentiate into all three germ layers via embryoid body and teratoma formation. These results suggest that autologous fibroblasts can be not only a source for iPS cells but also be feeder layers. Our results provide a possibility to solve the dilemma by using isogenic fibroblasts as feeder layers of iPS cells. This is an important step toward the establishment of clinical grade iPS cells.
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影响因子：
3.6
作者：
Park, JH;Kim, SJ;Yoon, HS
通讯作者：
Yoon, HS
影响因子：
6.1
作者：
Park, SP;Lee, YJ;Lim, JH
通讯作者：
Lim, JH
影响因子：
11.4
作者：
Yamanaka, S;Zhang, XY;Innerarity, TL
通讯作者：
Innerarity, TL
DOI：
10.1126/science.1172482
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Yu J;Hu K;Smuga-Otto K;Tian S;Stewart R;Slukvin II;Thomson JA
通讯作者：
Thomson JA
影响因子：
56.9
作者：
Yu, Junying;Vodyanik, Maxim A.;Thomson, James A.
通讯作者：
Thomson, James A.