In vivo Imaging of a Novel Strain of Bacteroides fragilis via Metabolic Labeling.
In vivo Imaging of a Novel Strain of Bacteroides fragilis via Metabolic Labeling.
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通过代谢标记对脆弱拟杆菌新菌株进行体内成像
DOI：
10.3389/fmicb.2018.02298
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发表时间：
2018
影响因子：
5.2
通讯作者：
Zhi F
中科院分区：
文献类型：
--
作者：
Xu W;Su P;Zheng L;Fan H;Wang Y;Liu Y;Lin Y;Zhi F
非产芽孢脆弱类杆菌因其诸多优点而被认为是一种潜在的益生菌候选菌。我们以前分离出一种新的B菌株。fragilis（ZY-312）的生物安全性和体内抑制病原体生长的能力。然而，ZY-312在胃肠道（GI）中的定殖仍有待确定。为了追踪ZY-312的定殖，小鼠灌胃通过代谢寡糖工程和生物正交点击化学标记的ZY-312或直接给予AF 647-二苯并环辛炔（DIBO）。然后在胃肠道、脾脏和肾脏中检测荧光。结果表明，ZY-312可被代谢寡糖工程标记，AF 647-DIBO体外最佳作用时间为5 h。在用AF 647-DIBO标记的ZY-312或单独的AF 647-DIBO经口管饲后，对小鼠进行体内成像，并且在管饲后3小时、6小时和12小时，两组中的荧光强度相似。AF 647-DIBO组的荧光在灌胃后24小时消失，这可能是由于通过胃肠道排泄。而AF 647-DIBO标记的ZY-312在盲肠中的荧光保留时间长达48 h。免疫荧光实验进一步证实，ZY-312不仅在盲肠内，而且在胃、回肠和结肠内也有短暂的定殖，在其他器官如肾脏和脾脏中未检测到ZY-312的大量蓄积。结论：ZY-312能在胃肠道以盲肠为主要部位短暂定植至少48 h，且几乎不向其他器官扩散，为B的进一步研究提供了新的思路。fragilis作为益生菌产品。
Non-toxigenic Bacteroides fragilis is regarded as a potential candidate for probiotic owing to its various advantages. We previously isolated a new strain of B. fragilis (ZY-312) and verified its biosafety and capability of inhibiting the growth of pathogens in vivo. However, the colonization of ZY-312 in gastrointestinal (GI) tract remains to be determined. To track the colonization of ZY-312, mice were gavaged with ZY-312 labeled by means of metabolic oligosaccharide engineering and bioorthogonal click chemistry or given AF647-dibenzocyclooctyne (DIBO) directly. Then the fluorescence was detected in GI tract, spleen and kidneys. Results showed that ZY-312 could be labeled by metabolic oligosaccharide engineering, and the optimal incubation time with AF647-DIBO was 5 h in vitro. Following oral gavage with AF647-DIBO labeled ZY-312 or AF647-DIBO alone, mice were subjected to in vivo imaging and the fluorescence intensity was similar in both groups 3 h, 6 h, and 12 h post the gavage. The fluorescence of AF647-DIBO group disappeared 24 h post gavage which was probably due to the excretion via GI tract. While the fluorescence of AF647-DIBO labeled ZY-312 retained in the cecum for as long as 48 h. Immunofluorescence assay further confirmed that labeled ZY-312 transiently colonized not only in cecum but also in stomach, ileum and colon of mice 48 h post-gavage and that no massive accumulation of ZY-312 was detected in other organs such as kidneys and spleen. In conclusion, ZY-312 could transiently colonize in GI tract, mainly in cecum, for at least 48 h, and it hardly disseminate to other organs, which shed new light on the future development of B. fragilis as a probiotic product.
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影响因子：
5.7
作者：
Li Z;Deng H;Zhou Y;Tan Y;Wang X;Han Y;Liu Y;Wang Y;Yang R;Bi Y;Zhi F
通讯作者：
Zhi F
Wnt1 在感染相关结直肠癌中的新调节作用
DOI：
10.1016/j.neo.2018.03.001
发表时间：
2018-05
期刊：
Neoplasia (New York, N.Y.)
影响因子：
--
作者：
Wang J;Lu R;Fu X;Dan Z;Zhang YG;Chang X;Liu Q;Xia Y;Liu X;Sun J
通讯作者：
Sun J
影响因子：
4
作者：
Dehnert, Karen W.;Beahm, Brendan J.;Huynh, Thinh T.;Baskin, Jeremy M.;Laughlin, Scott T.;Wang, Wei;Wu, Peng;Amacher, Sharon L.;Bertozzi, Carolyn R.
通讯作者：
Bertozzi, Carolyn R.
影响因子：
4.4
作者：
Ochoa-Reparaz, Javier;Mielcarz, Daniel W.;Kasper, Lloyd H.
通讯作者：
Kasper, Lloyd H.
影响因子：
2.8
作者：
Martin, Rebeca;Sanchez, Borja;Bermudez-Humaran, Luis G.
通讯作者：
Bermudez-Humaran, Luis G.