Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato.
Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato.
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DOI：
10.1016/j.simyco.2018.01.002
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发表时间：
2018-03
影响因子：
16.5
通讯作者：
Vleeshouwers VGAA
中科院分区：
文献类型：
--
作者：
Aguilera-Galvez C;Champouret N;Rietman H;Lin X;Wouters D;Chu Z;Jones JDG;Vossen JH;Visser RGF;Wolters PJ;Vleeshouwers VGAA
马铃薯晚疫病是由马铃薯晚疫病菌引起的一种危害最大的病害。对于这种经济上重要的疾病的可持续管理，抗药性育种依赖于抗药性(R)基因的可用性。这种抗马铃薯晚疫病菌的R基因在北美、中美洲和南美洲的野生块茎茄子物种中与同源无毒(AVR)基因共同进化而成。在这里，我们报告了用白粉菌Avr2的效应组学筛选如何揭示原产于墨西哥和秘鲁的不同茄子物种的防御反应。我们发现，墨西哥茄子对AVR2的反应是由R2家族的R基因介导的，该基因位于第四染色体上的一个主要晚疫病基因座上。相反，秘鲁茄子对AVR2的反应是由RPI-mcq1介导的，该基因位于第九染色体上，不属于R2家族。这些数据表明，在墨西哥和秘鲁的茄子中，AVR2的识别分别在两个遗传位点上独立进化。从R2或RPI-mcq1基因座转化R基因的马铃薯品种‘Désirée’的离体叶片试验显示，对18个不同的致病疫霉分离物具有重叠但不同的抗性图谱。在分子R-AVR基因相互作用方面所取得的研究成果将有助于更好地利用R基因，并最大限度地发挥产生更广谱、更持久控制马铃薯晚疫病的潜力。
Late blight, caused by the oomycete pathogen Phytophthora infestans, is the most devastating disease in potato. For sustainable management of this economically important disease, resistance breeding relies on the availability of resistance (R) genes. Such R genes against P. infestans have evolved in wild tuber-bearing Solanum species from North, Central and South America, upon co-evolution with cognate avirulence (Avr) genes. Here, we report how effectoromics screens with Avr2 of P. infestans revealed defense responses in diverse Solanum species that are native to Mexico and Peru. We found that the response to AVR2 in the Mexican Solanum species is mediated by R genes of the R2 family that resides on a major late blight locus on chromosome IV. In contrast, the response to AVR2 in Peruvian Solanum species is mediated by Rpi-mcq1, which resides on chromosome IX and does not belong to the R2 family. The data indicate that AVR2 recognition has evolved independently on two genetic loci in Mexican and Peruvian Solanum species, respectively. Detached leaf tests on potato cultivar ‘Désirée’ transformed with R genes from either the R2 or the Rpi-mcq1 locus revealed an overlapping, but distinct resistance profile to a panel of 18 diverse P. infestans isolates. The achieved insights in the molecular R – Avr gene interaction can lead to more educated exploitation of R genes and maximize the potential of generating more broad-spectrum, and potentially more durable control of the late blight disease in potato.
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影响因子：
2.9
作者：
Haverkort, A. J.;Boonekamp, P. M.;Visser, R. G. F.
通讯作者：
Visser, R. G. F.
影响因子：
4.5
作者：
Abad, ZG;Abad, JA
通讯作者：
Abad, JA
影响因子：
3.2
作者：
GOODWIN, SB;SPIELMAN, LJ;FRY, WE
通讯作者：
FRY, WE
影响因子：
3.5
作者：
DEFEYTER, R;YANG, YO;GABRIEL, DW
通讯作者：
GABRIEL, DW
DOI：
10.1073/pnas.0611479104
发表时间：
2007-02-27
影响因子：
11.1
作者：
Gomez-Alpizar, Luis;Carbone, Ignazio;Ristaino, Jean Beagle
通讯作者：
Ristaino, Jean Beagle