The nested embryonic dorsal domains of BMP-target genes are not scaled to size during the evolution of Drosophila species.
The nested embryonic dorsal domains of BMP-target genes are not scaled to size during the evolution of Drosophila species.
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DOI：
10.1002/jez.b.23137
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发表时间：
2023-03
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影响因子：
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卵的大小是果蝇中一个快速进化的特征，预计会改变胚胎轴的形态发生蛋白的空间分布。在这里，我们询问了十肢麻痹/骨形态发生蛋白-4（DPP/BMP-4）梯度对胚胎背部区域的图案化是否在具有不同卵大小的果蝇物种中缩放。该区域指定了胚外组织浆膜和外胚层。我们发现，整个背部区域的规模与胚胎的大小，但由DPP调控的基因表达模式是不成比例的，这表明DPP梯度在进化过程中的差异缩放。为了进一步测试DPP梯度是否可以在黑腹果蝇中扩展，我们创建了具有扩展的背侧区域的胚胎，这些区域模拟了在其他物种中观察到的规模变化，并测量了DPP靶基因的结果域。我们发现，这些领域的比例没有保持，这表明DPP梯度是无法在胚胎中扩展。这些和以前的研究结果表明，胚胎背腹侧图案缺乏腹侧和背侧的缩放，但在神经外胚层被指定的外侧区域是稳健的，并且两个相反的梯度，背侧/NF κ-B和DPP相交。我们建议，缺乏缩放的DPP梯度可能会导致浆膜的大小和外胚层细胞的数量与特定的皮质张力的变化，这是预计将产生不同的机械力，为不同大小的原肠胚。果蝇属物种背侧胚胎区域的图案并不按胚胎大小缩放。在具有扩展背侧区域的果蝇胚胎中，十肢麻痹（DPP）梯度不成比例。浆膜和外胚层域大小的进化变化应修改机械力在不同大小的胚胎原肠胚形成。不同卵大小的果蝇胚胎背部区域内基因表达结构域的表现。背侧区标记的十肢麻痹（dpp）表达扩大成比例与胚胎大小。然而，DPP-靶基因的表达结构域宽度的比率在黑腹果蝇和塞切利亚果蝇之间出现偏斜，表明DPP/BMP-4梯度在这两个物种中的比例存在差异。在D.黑腹果蝇的研究表明，当胚胎的背侧区域扩张时，这种梯度并不成比例。
Egg size is a fast‐evolving trait among Drosophilids expected to change the spatial distribution of morphogens that pattern the embryonic axes. Here we asked whether the patterning of the dorsal region of the embryo by the Decapentaplegic/Bone Morphogenetic Protein‐4 (DPP/BMP‐4) gradient is scaled among Drosophila species with different egg sizes. This region specifies the extra‐embryonic tissue amnioserosa and the ectoderm. We find that the entire dorsal region scales with embryo size, but the gene expression patterns regulated by DPP are not proportional, suggesting that the DPP gradient is differentially scaled during evolution. To further test whether the DPP gradient can scale or not in Drosophila melanogaster, we created embryos with expanded dorsal regions that mimic changes in scale seen in other species and measured the resulting domains of DPP‐target genes. We find that the proportions of these domains are not maintained, suggesting that the DPP gradient is unable to scale in the embryo. These and previous findings suggest that the embryonic dorso‐ventral patterning lack scaling in the ventral and dorsal sides but is robust in the lateral region where the neuroectoderm is specified and two opposing gradients, Dorsal/NFkappa‐B and DPP, intersect. We propose that the lack of scaling of the DPP gradient may contribute to changes in the size of the amnioserosa and the numbers of ectodermal cells with specific cortical tensions, which are expected to generate distinct mechanical forces for gastrulating embryos of different sizes. The patterning of the dorsal embryonic region of Drosophila species are not scaled to embryonic size. The Decapentaplegic (DPP) gradient does not scale in Drosophila melanogaster embryos with expanded dorsal region. Evolutionary changes in amnioserosa and ectodermal domain sizes should modify mechanical forces during gastrulation of embryos of different sizes. Representation of gene expression domains within the dorsal region of embryos from Drosophila species that vary in egg size. The dorsal region marked by decapentaplegic (dpp) expression expands proportionally with embryo size. However, the ratios of expression domain widths of DPP‐target genes appear skewed between Drosophila melanogaster and Drosophila sechellia, suggesting that the DPP/BMP‐4 gradients scale differentially within these two species. Additional experiments in D. melanogaster show that this gradient does not scale when the dorsal region of the embryo is expanded.
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DOI：
10.1073/pnas.0509483102
发表时间：
2005-12-20
影响因子：
11.1
作者：
Gregor, T;Bialek, W;Wieschaus, EF
通讯作者：
Wieschaus, EF
DOI：
10.1073/pnas.0806476105
发表时间：
2008-12-23
影响因子：
11.1
作者：
Hong, Joung-Woo;Hendrix, David A.;Levine, Michael S.
通讯作者：
Levine, Michael S.
影响因子：
11.4
作者：
JIMENEZ, F;MARTINMORRIS, LE;WHITE, K
通讯作者：
WHITE, K
影响因子：
64.8
作者：
Ashe, HL;Levine, M
通讯作者：
Levine, M
影响因子：
10.5
作者：
FRANCOIS, V;SOLLOWAY, M;BIER, E
通讯作者：
BIER, E