The (13)C hyperpolarized pyruvate generated by ParaHydrogen detects the response of the heart to altered metabolism in real time.
The (13)C hyperpolarized pyruvate generated by ParaHydrogen detects the response of the heart to altered metabolism in real time.
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DOI：
10.1038/s41598-018-26583-2
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发表时间：
2018-05-30
影响因子：
4.6
通讯作者：
Reineri F
中科院分区：
文献类型：
--
作者：
Cavallari E;Carrera C;Sorge M;Bonne G;Muchir A;Aime S;Reineri F
已经提出了许多成像方法作为器官损伤的替代标志物，但对于许多候选人，受伤器官的基本生物标志物特征尚未被描述。超极化的[1- 13 C]丙酮酸盐允许真实的体内代谢监测。仲氢诱导极化（PHIP）是一种便携式的，具有成本效益的技术，能够在几秒钟内产生13 C MR超极化分子。侧臂氢化（SAH）策略的引入提供了一种拓宽PHIP生成系统领域的方法，并使这种方法与目前应用的溶解-DNP（动态核极化）方法竞争。在此，我们描述了第一个使用PHIP-SAH超极化[1- 13 C]丙酮酸盐的体内代谢成像研究。丙酮酸及其代谢产物乳酸的体内图谱已在1 T MRI扫描仪上获得。通过比较扩张型心肌病小鼠模型中丙酮酸/乳酸13 C标记交换率，已经发现，在通过超声心动图研究评估疾病之前，可以很好地检测到患病小鼠心肌中发生的代谢功能障碍。
Many imaging methods have been proposed to act as surrogate markers of organ damage, yet for many candidates the essential biomarkers characteristics of the injured organ have not yet been described. Hyperpolarized [1-13C]pyruvate allows real time monitoring of metabolism in vivo. ParaHydrogen Induced Polarization (PHIP) is a portable, cost effective technique able to generate 13C MR hyperpolarized molecules within seconds. The introduction of the Side Arm Hydrogenation (SAH) strategy offered a way to widen the field of PHIP generated systems and to make this approach competitive with the currently applied dissolution-DNP (Dynamic Nuclear Polarization) method. Herein, we describe the first in vivo metabolic imaging study using the PHIP-SAH hyperpolarized [1-13C]pyruvate. In vivo maps of pyruvate and of its metabolic product lactate have been acquired on a 1 T MRI scanner. By comparing pyruvate/lactate 13C label exchange rate in a mouse model of dilated cardiomyopathy, it has been found that the metabolic dysfunction occurring in the cardiac muscle of the diseased mice can be detected well before the disease can be assessed by echocardiographic investigations.
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影响因子：
3.5
作者：
Arimura, T;Helbling-Leclerc, A;Bonne, G
通讯作者：
Bonne, G
影响因子：
3.3
作者：
Bastiaansen JAM;Yoshihara HAI;Capozzi A;Schwitter J;Gruetter R;Merritt ME;Comment A
通讯作者：
Comment A
影响因子：
82.9
作者：
Day, Sam E.;Kettunen, Mikko I.;Brindle, Kevin M.
通讯作者：
Brindle, Kevin M.
影响因子：
3.7
作者：
Hillemann, Doris;Hoffner, Sven;Ruesch-Gerdes, Sabine
通讯作者：
Ruesch-Gerdes, Sabine
DOI：
10.1007/s10334-008-0155-x
发表时间：
2009-04-01
影响因子：
2.3
作者：
Hoevener, Jan-Bernd;Chekmenev, Eduard Y.;Bhattacharya, Pratip
通讯作者：
Bhattacharya, Pratip