The isolation and sequence of a novel gene from a human functional T cell line.
The isolation and sequence of a novel gene from a human functional T cell line.
复制标题
从人类功能T细胞系中新型基因的分离和序列。
DOI：
10.1084/jem.165.3.601
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发表时间：
1987-03-01
影响因子：
15.3
通讯作者：
KRENSKY, AM
中科院分区：
文献类型：
--
作者：
JONGSTRA, J;SCHALL, TJ;DYER, BJ;CLAYBERGER, C;JORGENSEN, J;DAVIS, MM;KRENSKY, AM
使用消减杂交程序，我们已经构建了一个cDNA文库，该文库富含功能性人T细胞系中存在的序列，但不富含人EBV转化的B细胞系中存在的序列。我们已经分离了一个cDNA克隆，AH 2 -519，代表一个新的基因，命名为519。这种新的基因在功能性人溶细胞和Th细胞系中表达，但在多种其他细胞系中不表达，包括几种长期的人T细胞肿瘤系。基因519的表达在正常人PBL的培养物中使用抗原或促有丝分裂刺激是可诱导的。从与克隆AH 2 -519重叠的全长cDNA克隆确定的DNA序列和其预测蛋白产物的氨基酸序列与GenBank或NBRF数据库中公布的序列都没有显著的同源性。基因519的限制性表达表明其基因产物参与正常T细胞的生长和/或分化。这些数据还表明，正常的、非转化的、功能性T细胞表达的基因产物在相同细胞谱系的长期肿瘤系中不能容易地被识别。
Using a subtractive hybridization procedure we have constructed a cDNA library enriched for sequences present in functional human T cell lines, but not in human EBV-transformed B cell lines. We have isolated a cDNA clone, AH2-519, representing a novel gene, designated 519. This novel gene is expressed in functional human cytolytic and Th cell lines but not in a variety of other cell lines, including several long-term human T cell tumor lines. The expression of gene 519 is inducible in cultures of normal human PBL using antigenic or mitogenic stimulation. Neither the DNA sequence determined from a full-length cDNA clone overlapping with clone AH2-519 nor the amino acid sequence of its predicted protein product has significant homology to published sequences in the GenBank or NBRF databases. The restricted expression of gene 519 suggests that its gene product is involved in the growth and/or differentiation of normal T cells. The data also show that normal, nontransformed, functional T cells express gene products that can not be readily identified in long-term tumor lines of the same cell lineage.
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影响因子：
56.9
作者：
GERSHENFELD, HK;WEISSMAN, IL
通讯作者：
WEISSMAN, IL
影响因子：
64.5
作者：
FUJITA, T;SHIBUYA, H;TANIGUCHI, T
通讯作者：
TANIGUCHI, T
DOI：
10.1073/pnas.81.7.2194
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DAVIS, MM;COHEN, DI;HOOD, L
通讯作者：
HOOD, L
影响因子：
64.8
作者：
GILLIS, S;SMITH, KA
通讯作者：
SMITH, KA
DOI：
10.1073/pnas.82.6.1673
发表时间：
1985-01-01
影响因子：
11.1
作者：
KAHANA, C;NATHANS, D
通讯作者：
NATHANS, D