CD147 promotes cell motility via upregulation of p190-B RhoGAP in hepatocellular carcinoma.
CD147 promotes cell motility via upregulation of p190-B RhoGAP in hepatocellular carcinoma.
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CD147 通过上调 p190-B RhoGAP 在肝细胞癌中促进细胞运动
DOI：
10.1186/s12935-016-0344-z
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发表时间：
2016
影响因子：
5.8
通讯作者：
Cui HY
中科院分区：
文献类型：
--
作者：
Chen R;Wang SJ;Zhang Y;Hou R;Jiang JL;Cui HY
BackgroundThe acquisition of inappropriate migratory feature is crucial for tumor metastasis. Rho-family GTPases including RhoA are molecular switches that play critical roles in regulating cell movement. We investigated the molecular mechanism underlying CD147 induced RhoA deactivation in hepatocellular carcinoma (HCC) cells.MethodsWound-healing assay was performed to study the cell motility. Analysis of RhoA activation in living cells was conducted using RhoA biosensor. Changes in the expression of certain genes were determined by quantitative real-time PCR. The expression of proteins was evaluated by Western blot. Cytoskeleton reorganization and focal adhesion formation were observed by immunofluorescence staining. Further investigation on the correlation between CD147 and p190-B RhoGAP (p190-B) in HCC tissues was performed by immunological histological chemistry analysis.ResultsCD147 promoted cell movement and suppressed RhoA activation. p190-B, a negative regulator of RhoA activity, was upregulated by CD147 at both mRNA and protein levels. This regulatory relationship was further confirmed by analyzing the expression pattern of CD147 and p190-B in human HCC tissues. Silencing of p190-B caused the increased formation of stress fiber and focal adhesion and blunted the impact of CD147 overexpression on cell movement, indicating that the regulatory effect of CD147 on cell movement is mediated, at least partially, by p190-B.ConclusionsThese findings indicated that p190-B, a negative regulator of RhoA, is positively regulated by CD147 and contributes to the regulation of cell movement in HCC. CD147 plays critical roles in the motility of cancer cells and may be therefore a valuable drug target for anti-cancer therapy.
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HAb18G/CD147 调节培养的人肝细胞癌细胞中粘着蛋白介导的局部粘附和细胞骨架组织
影响因子：
3.7
作者：
Liang Q;Han Q;Huang W;Nan G;Xu BQ;Jiang JL;Chen ZN
通讯作者：
Chen ZN
影响因子：
--
作者：
Kieliszek M;Błażejak S;Bzducha-Wróbel A
通讯作者：
Bzducha-Wróbel A
影响因子：
64.8
作者：
Pertz, O;Hodgson, L;Hahn, KM
通讯作者：
Hahn, KM
影响因子：
3.9
作者：
Ru, Ning-Yu;Wu, Jiao;Bian, Huijie
通讯作者：
Bian, Huijie
影响因子：
4.8
作者：
Li, Yong;Wu, Jiao;Jiang, Jian-Li
通讯作者：
Jiang, Jian-Li