High resolution analysis of DNA copy-number aberrations of chromosomes 8, 13, and 20 in gastric cancers.
High resolution analysis of DNA copy-number aberrations of chromosomes 8, 13, and 20 in gastric cancers.
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DOI：
10.1007/s00428-009-0814-y
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发表时间：
2009-09
期刊：
影响因子：
--
通讯作者：
Meijer GA
中科院分区：
文献类型：
--
作者：
Buffart TE;van Grieken NC;Tijssen M;Coffa J;Ylstra B;Grabsch HI;van de Velde CJ;Carvalho B;Meijer GA
染色体8 q、13 q和20 q的DNA拷贝数增加经常在胃癌中观察到。此外，染色体20 q的增加与淋巴结转移有关。本研究的目的是探讨胃腺癌中染色体8 q、13 q和20 q上单个基因的DNA拷贝数变化与临床病理资料的关系。通过全基因组微阵列比较基因组杂交和多重连接依赖性探针扩增（MLPA）分析了从63例福尔马林固定和石蜡包埋的胃腺癌组织样本中分离的DNA，靶向8、13和20号染色体上的58个基因。采用阵列比较基因组杂交技术，分别在49例（77.8%）、25例（39.7%）和49例（77.8%）胃腺癌中观察到8 q、13 q和20 q的增加。染色体20 q的增加与淋巴结转移（p = 0.05）和组织学类型（p = 0.02）显著相关。MLPA揭示了几个基因的DNA拷贝数经常获得。癌基因c-myc在8 q上的阳性率为73%，而FOXO 1A和ATP 7 B在13 q上的阳性率为28.6%。染色体20 q上的多个基因在超过60%的癌症中显示出增益。TNFRSF 6 B（20q13.3）和ZNF 217（20q13.2）的DNA拷贝数增加分别与淋巴结转移（p = 0.02）和组织学类型（p = 0.02）显著相关。总之，胃腺癌中染色体8 q、13 q和20 q的增加携带已知和推定癌基因的DNA拷贝数增加。ZNF 217和TNFRSF 6 B与重要的临床病理变量相关，包括淋巴结状态。
DNA copy-number gains of chromosomes 8q, 13q, and 20q are frequently observed in gastric cancers. Moreover gain of chromosome 20q has been associated with lymph node metastasis. The aim of this study was to correlate DNA copy-number changes of individual genes on chromosomes 8q, 13q, and 20q in gastric adenocarcinomas to clinicopathological data. DNA isolated from 63 formalin-fixed and paraffin-embedded gastric adenocarcinoma tissue samples was analyzed by whole-genome microarray comparative genomic hybridization and by multiplex ligation-dependent probe amplification (MLPA), targeting 58 individual genes on chromosomes 8, 13, and 20. Using array comparative genomic hybridization, gains on 8q, 13q, and 20q were observed in 49 (77.8%), 25 (39.7%), and 49 (77.8%) gastric adenocarcinomas, respectively. Gain of chromosome 20q was significantly correlated with lymph node metastases (p = 0.05) and histological type (p = 0.02). MLPA revealed several genes to be frequently gained in DNA copy number. The oncogene c-myc on 8q was gained in 73% of the cancers, while FOXO1A and ATP7B on 13q were both gained in 28.6% of the cases. Multiple genes on chromosome 20q showed gains in more than 60% of the cancers. DNA copy-number gains of TNFRSF6B (20q13.3) and ZNF217 (20q13.2) were significantly associated with lymph node metastasis (p = 0.02) and histological type (p = 0.02), respectively. In summary, gains of chromosomes 8q, 13q, and 20q in gastric adenocarcinomas harbor DNA copy-number gains of known and putative oncogenes. ZNF217 and TNFRSF6B are associated with important clinicopathological variables, including lymph node status.
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影响因子：
1.9
作者：
Aust, DE;Muders, M;Baretton, GB
通讯作者：
Baretton, GB
影响因子：
5.8
作者：
van de Wiel, Mark A.;Kim, Kyung In;Ylstra, Bauke
通讯作者：
Ylstra, Bauke
DOI：
10.1111/apm.1965.64.1.31
发表时间：
1965-01-01
期刊：
ACTA PATHOLOGICA ET MICROBIOLOGICA SCANDINAVICA
影响因子：
--
作者：
LAUREN, P
通讯作者：
LAUREN, P
影响因子：
--
作者：
Kokkola, A;Monni, O;Knuutila, S
通讯作者：
Knuutila, S
影响因子：
7.3
作者：
Buffart, T. E.;Carvalho, B.;Meijer, G. A.
通讯作者：
Meijer, G. A.