Iron availability increases the pathogenic potential of Salmonella typhimurium and other enteric pathogens at the intestinal epithelial interface.
Iron availability increases the pathogenic potential of Salmonella typhimurium and other enteric pathogens at the intestinal epithelial interface.
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DOI：
10.1371/journal.pone.0029968
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Tjalsma H
中科院分区：
文献类型：
--
作者：
Kortman GA;Boleij A;Swinkels DW;Tjalsma H
最近的试验质疑了未限制的口服铁的安全性。 Teria在下面培养铁限制的条件以及浓度增加的柠檬酸盐，以评估细菌的影响，上皮粘附，侵袭，易位和上皮损伤的体外易位和上皮完整性实验。其他促进病原体增加对铁链球菌对上皮细胞的粘附，当这些细菌与铁浓度增加时，这些细菌的粘附力显着增加（p = 0.0001），而非疾病的乳杆菌属于plotiratiral traptim trimial trimial trimial trimial trimial trimial thrimitial thrimitial trimistiral thrimitial thrimitial thrimiate s.伤寒或在富含铁的条件下预孵育的柠檬酸素食。
Recent trials have questioned the safety of untargeted oral iron supplementation in developing regions. Excess of luminal iron could select for enteric pathogens at the expense of beneficial commensals in the human gut microflora, thereby increasing the incidence of infectious diseases. The objective of the current study was to determine the effect of high iron availability on virulence traits of prevalent enteric pathogens at the host-microbe interface. A panel of enteric bacteria was cultured under iron-limiting conditions and in the presence of increasing concentrations of ferric citrate to assess the effect on bacterial growth, epithelial adhesion, invasion, translocation and epithelial damage in vitro. Translocation and epithelial integrity experiments were performed using a transwell system in which Caco-2 cells were allowed to differentiate to a tight epithelial monolayer mimicking the intestinal epithelial barrier. Growth of Salmonella typhimurium and other enteric pathogens was increased in response to iron. Adhesion of S. typhimurium to epithelial cells markedly increased when these bacteria were pre-incubated with increasing iron concentration (P = 0.0001), whereas this was not the case for the non-pathogenic Lactobacillus plantarum (P = 0.42). Cellular invasion and epithelial translocation of S. typhimurium followed the trend of increased adhesion. Epithelial damage was increased upon incubation with S. typhimurium or Citrobacter freundii that were pre-incubated under iron-rich conditions. In conclusion, our data fit with the consensus that oral iron supplementation is not without risk as iron could, in addition to inducing pathogenic overgrowth, also increase the virulence of prevalent enteric pathogens.
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