Trithorax-group protein ATX5 mediates the glucose response via impacting the HY1-ABI4 signaling module
Trithorax-group protein ATX5 mediates the glucose response via impacting the HY1-ABI4 signaling module
复制标题
Trithorax 组蛋白 ATX5 通过影响 HY1-ABI4 信号模块介导葡萄糖反应
DOI：
10.1007/s11103-018-0791-0
复制
发表时间：
2018-11
影响因子：
5.1
通讯作者：
Xu Zheng-Yi
中科院分区：
文献类型：
--
作者：
Liu Yutong;Wang Jie;Yin Hao;Zhang Ai;Huang Shuangzhan;Wang Tian-Jing;Meng Qingxiang;Nan Nan;Wu Yifan;Guo Peng;Ahmad Rafiq;Liu Bao;Xu Zheng-Yi
拟南芥TRITHORAX5蛋白调控葡萄糖反应。摘要葡萄糖是一种从单细胞微生物到多细胞动物和植物的进化保守调节剂。大量研究表明，Trithorax-group蛋白（TrxGs）通过影响组蛋白修饰和染色质结构，在不同的生物过程中发挥重要作用。然而，TrxGs是否在葡萄糖反应中起作用，以及它们如何在植物中实现对葡萄糖信号的靶基因的控制，仍然是未知的。在这里，我们发现拟南芥TRITHORAX5蛋白（ATX5）影响葡萄糖反应和信号传导。与野生型（WT）相比，atx5功能缺失突变体表现出葡萄糖过度敏感的表型。全基因组rna测序分析显示，ATX5影响葡萄糖信号应答基因子集的表达。有趣的是，我们已经确定ATX5通过三甲基化拟南芥氧合酶1（HY1）位点的H3赖氨酸4直接控制HY1的表达。葡萄糖信号导致ATX5活性的抑制，随后降低hy1位点的H3K4me3水平，从而导致abscisic ACID-INSENSITIVE4（ABI4）的表达增加。这一结果表明，一个重要的atx5 - hy1 - abi4调节模块控制着葡萄糖反应。遗传证据进一步支持了这一观点，表明anatx5 hy1-100 abi4三突变体与abi4单突变体相比表现出相似的葡萄糖不敏感表型。我们的研究结果表明，一种新的ATX5-HY1-ABI4模块控制拟南芥的葡萄糖反应。
Key messageTrithorax-group Protein ARABIDOPSIS TRITHORAX5 modulates the glucose response.AbstractGlucose is an evolutionarily conserved modulator from unicellular microorganisms to multicellular animals and plants. Extensive studies have shown that the Trithorax-group proteins (TrxGs) play essential roles in different biological processes by affecting histone modifications and chromatin structures. However, whether TrxGs function in the glucose response and how they achieve the control of target genes in response to glucose signaling in plants remain unknown. Here, we show that the Trithorax-group Protein ARABIDOPSIS TRITHORAX5 (ATX5) affects the glucose response and signaling.atx5loss-of-function mutants display glucose-oversensitive phenotypes compared to the wild-type (WT). Genome-wide RNA-sequencing analyses have revealed that ATX5 impacts the expression of a subset of glucose signaling responsive genes. Intriguingly, we have established that ATX5 directly controls the expression ofHY1by trimethylating H3 lysine 4 of the ArabidopsisHeme Oxygenase1(HY1) locus. Glucose signaling causes the suppression of ATX5 activity and subsequently reduces the H3K4me3 levels at theHY1locus, thereby leading to the increased expression ofABSCISIC ACID-INSENSITIVE4(ABI4). This result suggests that an importantATX5-HY1-ABI4regulatory module governs the glucose response. This idea is further supported by genetic evidence showing that anatx5 hy1-100 abi4 triplemutant showed a similar glucose-insensitive phenotype as compared to that of theabi4single mutant. Our findings show that a novel ATX5-HY1-ABI4 module controls the glucose response inArabidopsis thaliana.
登录
查看更多内容
影响因子：
2.2
作者：
Shen, Ya-Ching;Liaw, Chia-Ching;Kuo, Yau-Haur
通讯作者：
Kuo, Yau-Haur
影响因子：
7.3
作者：
Kim, Young-Min;Heinzel, Nicolas;Hajirezaei, Mohammad-Reza
通讯作者：
Hajirezaei, Mohammad-Reza
影响因子：
6.9
作者：
Cao Z;Geng B;Xu S;Xuan W;Nie L;Shen W;Liang Y;Guan R
通讯作者：
Guan R
影响因子：
3.4
作者：
Veerappan CS;Avramova Z;Moriyama EN
通讯作者：
Moriyama EN
影响因子：
5.2
作者：
Han, Bin;Xu, Sheng;Xie, Gui-Shui
通讯作者：
Xie, Gui-Shui