Production of subgroup‐specific monoclonal antibodies against human rotaviruses and their application to an enzyme‐linked immunosorbent assay for subgroup determination

Production of subgroup‐specific monoclonal antibodies against human rotaviruses and their application to an enzyme‐linked immunosorbent assay for subgroup determination
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抗人轮状病毒亚组特异性单克隆抗体的制备及其在酶联免疫吸附测定亚组测定中的应用

DOI:
10.1002/jmv.1890140205
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发表时间:
1984
影响因子:
12.7
通讯作者:
Toshio Yasuhara
Toshio Yasuhara
中科院分区:
医学3区
文献类型:
--
作者:
K. Taniguchi;T. Urasawa;S. Urasawa;Toshio Yasuhara

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非中和性单克隆抗体的制备对两个菌株,S2和YO,人轮状病毒分离的细胞培养。S2 - 37和YO-5抗体分别具有亚组I和亚组II特异性。其余抗体(S2 - 65、YO-71、YO-89和YO-156)与所有检查的轮状病毒共同反应。所有单克隆抗体仅凝集单壳颗粒,并免疫沉淀42,000道尔顿蛋白质(内部衣壳的主要组分)。
Nonneutralizing monoclonal antibodies were prepared against two strains, S2 and YO, of human rotaviruses isolated in cell culture. S2‐37 and YO‐5 antibodies had subgroup I and subgroup II specificities, respectively. The remaining antibodies (S2‐65, YO‐71, YO‐89, and YO‐156) reacted commonly with all the rotaviruses examined. All of the monoclonal antibodies agglutinated exclusively single‐shelled particles and immunoprecipitated 42,000‐dalton protein, a major component of inner capsid.
DOI: 10.1016/0042-6822(81)90533-x
发表时间: 1981-01-01
期刊: VIROLOGY
影响因子: 3.7
作者:
LEE, PWK;HAYES, EC;JOKLIK, WK
通讯作者: JOKLIK, WK
使用单克隆抗体对单纯疱疹病毒进行血清分型的快速酶免疫过滤技术。
DOI: 10.1002/jmv.1890090408
发表时间: 1982
影响因子: 12.7
作者:
Richman,DD;Cleveland,PH;Oxman,MN
通讯作者: Oxman,MN